敏咳方通过Wnt/β-catenin信号通路及免疫因子动态变化对哮喘大鼠气道上皮屏障修复作用的研究  

Study on effect of Minke decoction on airway epithelial barrier repair in asthmatic rats through Wnt/β-catenin signaling pathway and dynamic changes of immune factors

作　　者：许洋洋 王文丽[2] XU Yang-yang;WANG Wen-li(Liaoning University of Traditional Chinese Medicine,Shenyang 110000,China)

机构地区：[1]辽宁中医药大学,110000 [2]辽宁中医药大学附属医院儿科,110000

出　　处：《中国实用医药》2023年第19期173-176,共4页China Practical Medicine

基　　金：辽宁省教育厅2019年科研项目(项目编号:L201904);辽宁省第一批中医优秀人才项目。

摘　　要：目的研究敏咳方通过分泌型糖蛋白/β-连环蛋白(Wnt/β-catenin)信号通路及免疫因子动态变化对哮喘大鼠气道上皮屏障的修复作用。方法选取36只无特定病原体级健康雄性SD大鼠,随机分为正常对照组、模型组、激素治疗组、敏咳方治疗组,每组9只。正常对照组大鼠不施加任何干预因素,自由食水;模型组大鼠采用卵清蛋白氢氧化铝溶液致敏并激发,再增加烟熏、激怒、高脂饮食等形成风痰瘀型;激素治疗组大鼠在模型组基础上加激素进行治疗;敏咳方治疗组大鼠在模型组基础上加敏咳方进行治疗。采用图像分析技术测定四组大鼠支气管管壁厚度、支气管平滑肌厚度;酶联免疫吸附测定(ELISA)法检测大鼠血清卵清蛋白特异性免疫球蛋白E(OVA-sIgE)水平,实时荧光定量聚合酶链反应(qRT-PCR)法测定无翅型MMTV整合位点家族成员7B(Wnt7B)、β-catenin的表达。比较四组大鼠Wnt7B、β-catenin及支气管管壁厚度、支气管平滑肌厚度、OVA-sIgE水平。结果正常对照组Wnt7B、β-catenin分别为(1.00±0.01)、(1.00±0.01),模型组分别为(10.03±1.23)、(1.95±0.27),激素治疗组分别为(5.32±0.42)、(1.42±0.18),敏咳方治疗组分别为(4.93±1.03)、(1.33±0.21);模型组Wnt7B、β-catenin的表达均大于正常对照组,差异有统计学意义(P<0.05);激素治疗组Wnt7B、β-catenin的表达均小于模型组,差异有统计学意义(P<0.05);敏咳方治疗组Wnt7B、β-catenin的表达均小于激素治疗组,差异有统计学意义(P<0.05)。正常对照组支气管管壁厚度、支气管平滑肌厚度及OVA-sIgE水平分别为(27.44±2.92)μm^(2)/μm、(12.58±0.99)μm^(2)/μm、(32.12±3.92)ng/ml,模型组分别为(58.12±7.71)μm^(2)/μm、(25.38±1.56)μm^(2)/μm、(117.22±19.21)ng/ml,激素治疗组分别为(41.51±3.39)μm^(2)/μm、(22.27±0.91)μm^(2)/μm、(67.59±7.92)ng/ml,敏咳方治疗组分别为(37.12±3.21)μm^(2)/μm、(20.11±0.89)μm^(2)/μm、(59.51±6.39)ng/ml�Objective To study on effect of Minke decoction on airway epithelial barrier repair in asthmatic rats through Wnt/β-catenin signaling pathway and dynamic changes of immune factors.Methods A total of 36 healthy male SD rats without specific pathogens were randomly divided into normal control group,model group,hormone treatment group and Minke decoction treatment group,with 9 rats in each group.Normal control group SD rats were given free water without any intervention factors;rats in model group were sensitized and stimulated with ovalbumin-aluminum hydroxide solution,then given smoke,irritability and high-fat diet to contribute wind phlegm stasis tye;rats in hormone treatment group were treated with hormone on the basis of model group;rats in Minke decoction treatment group were treated with Minke decoction on the basis of model group.The bronchial wall thickness and bronchial smooth muscle thickness were measured by image analysis;serum ovalbumin specific immunoglobulin E(OVA-SIGE)levels were measured by enzyme linked immunosorbent assay(ELISA);the expressions of wingless-type MMTV integration site family,member 7B(Wnt7B)andβ-catenin were measured by quantitative real-time reverse transcriptase polymerase chain reaction(qRT-PCR).The levels of Wnt7B,β-catenin,bronchial tube wall thickness,bronchial smooth muscle thickness and OVA-sIgE in the four groups were compared.Results The Wnt7B andβ-catenin were(1.00±0.01)and(1.00±0.01)in normal control group,(10.03±1.23)and(1.95±0.27)in model group,(5.32±0.42)and(1.42±0.18)in hormone treatment group,and(4.93±1.03)and(1.33±0.21)in Minke decoction treatment group.The expressions of Wnt7B andβ-catenin in model group were higher than those in normal control group,and the differences were statistically significant(P<0.05).The expressions of Wnt7B andβ-catenin in hormone treatment group were lower than those in model group,and the differences were statistically significant(P<0.05).The expressions of Wnt7B andβ-catenin in Minke decoction treatment group were lower

关 键 词：敏咳方 分泌型糖蛋白/β-连环蛋白信号通路 免疫因子 哮喘 

分 类 号：R285.5[医药卫生—中药学]