Preparation of bowl-shaped polydopamine surface imprinted polymer composite adsorbent for specific separation of 2'-deoxyadenosine  

作　　者：Pan Wang Mengdei Zhou Zhuangxin Wei Lu Liu Tao Cheng Xiaohua Tian Jianming Pan 

机构地区：[1]School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China

出　　处：《Chinese Journal of Chemical Engineering》2023年第8期69-79,共11页中国化学工程学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (22078132 and 22108103);Open Funding Project of the National Key Laboratory of Biochemical Engineering (2021KF-02);China Postdoctoral Science Foundation (2021M691301);Jiangsu Agricultural Independent Innovation Fund Project (CX(21)3079);Graduate Research Innovation Program of Jiangsu Province (KYCX20-3040);China Postdoctoral Science Foundation (2021M691301)。

摘　　要：Molecularly imprinted polymers (MIPs) have great potential as adsorbents for selective adsorption and separation of nucleoside compounds,but effectively enhancing the affinity of recognition sites by adjusting the forces between template molecules and functional monomers remains an important challenge.In this work,a surface imprinting strategy was used to construct bowl-shaped molecularly imprinted composite sorbents (BHPN@MIPs) based on polydopamine (PDA) particles and have achieved selective separation and purification of 2'-deoxyadenosine (dA).Where by the base complementary pairing interaction of the combined template molecule d A and the pyrimidine functional monomer can enhance the preassembly force,and the hydrophilic bowl-shaped PDA can provide a larger storage space contact efficiency of d A in the test solution,causing the site utilization much higher and improving the kinetic adsorption performance.The equilibrium adsorption time and maximum adsorption capacity of60 min and 328.45μmol·g^(-1)were observed by static adsorption experiments,and the selectivity experimental results showed an imprinting factor IF of 1.30.After four adsorption–desorption cycles,the initial adsorption equilibrium adsorption capacity of BHPN@MIPs still retained 91.14%.By evaluating the selective adsorption of d A in spiked human serum solutions,BHPN@MIPs can be used to selectively enrich and analyze target d A in complex biological samples.

关 键 词：Molecularly imprinted polymers(MIPs) Bowl-shaped Base complementary pairing interaction Selective separation 2'-deoxyadenosine(dA) 

分 类 号：R318[医药卫生—生物医学工程] TQ424[医药卫生—基础医学]