Reduced pigmentation and thyroid hormone disruption in zebrafish embryos caused by industrial sludge near Bohai Bay,China  

作　　者：Wenjing Dong Xiaoyu Yin Chelimuge Qi Tingting Wei Lijia Wei Jingfeng Yang Jingli Mu Hiroki Teraoka Wu Dong 

机构地区：[1]College of Animal Science and Technology,Inner Mongolia Minzu University/Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology,Tongliao,028000,China [2]School of Veterinary Medicine,Rakuno Gakuen University,Ebetsu,069-8501,Japan [3]College of Geography and Oceanography,Minjiang University,Fuzhou,350108,China

出　　处：《Water Biology and Security》2023年第4期36-44,共9页水生生物与安全（英文）

基　　金：funded by the National Natural Science Foundation of China(82060672);the Program for Interdisciplinary Innovation team in Inner Mongolia Minzu University,China(Dongwu-2023);the Natural Science Foundation of Inner Mongolia Autonomous Region of China(2020MS08103).

摘　　要：In recent years,pollution caused by the discharge of industrial wastewater into Bohai Bay has posed a potential threat to the health of surrounding residents.Sludge was collected from the outlet of a factory that discharges effluent into Bohai Bay,and alcohol extracts of sludge(SE)were prepared.We confirmed by UPLC-MS/MS analysis that the SE contained PAHs,including fluorene,pyrene,and phenanthrene.Zebrafish embryos as animal models were exposed to 0.1,0.3,0.5,1 and 5 mg/mL SE from 2 to 4 h post-fertilization(hpf)until 120 hpf.The results showed that SE caused a concentration-dependent increase in mortality and a decrease in hatchability.We found that SE significantly reduced eye pigmentation and decreased the movement of embryos and larvae.In addition,SE decreased triiodothyronine(T3)content and down-regulated the mRNA expression of some thyroid hormone-related genes including TPO and Thrβ,and caused the up-regulation of Dio2 and Dio3 at 120 hpf.Exposure to three individual PAHs found in SE,namely fluorene,pyrene,and phenanthrene,caused morphological and transcriptional changes that were similar to those caused by SE exposure.These findings indicate that PAHs in SE can reduce the pigmentation of zebrafish,which may be related to the genetic changes associated with thyroid hormones,and that zebrafish eye pigmentation can be used as an indicator of PAHs exposure.

关 键 词：Bohai bay PAHS PIGMENTATION THYROXINE Zebrafish embryos 

分 类 号：Q178.1[生物学—水生生物学]