How to use live sampling tissues and archived specimens in cetacean stable isotope research  

作　　者：Tao Jin Ruilong Wang Renyong Wang Jiayi Xie Jinsong Zheng Fei Fan Kexiong Wang Ding Wang Jun Xu Zhigang Mei 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology of China,Institute of Hydrobiology,Chinese Academy of Sciences,7 South Donghu Road,Wuchang District,Wuhan,430072,PR China [2]University of Chinese Academy of Sciences,19 Yuquan Road,Shijingshan District,Beijing,100049,PR China [3]Shanghai Aquatic Wildlife Conservation and Research Center,63 Chifeng Road,Yangpu District,Shanghai,200092,PR China

出　　处：《Water Biology and Security》2023年第4期61-68,共8页水生生物与安全（英文）

基　　金：supported by the National Key R&D Program of China(grant No.2018YFD0900904,2022YFF1301603);the INTERNATIONAL COOPERATION Project of the Chinese Academy of Sciences(Grant No.152342KYSB20190025).

摘　　要：Cetaceans are unique ecological engineers,and their restoration may have a crucial impact on the future structure of aquatic ecosystems,which calls for more investigations into their trophic ecology.Among current techniques,stable isotope analysis(SIA)has the advantages of non-invasive sampling and long timescales.However,the full benefits of SIA in cetacean research may not be achieved due to issues like different types of tissue between sampling methods and use of chemical preservation solutions in historical specimens.To address these challenges,we conducted a study on Narrow-ridged Finless Porpoises(Neophocaena asiaeorientalis).Multiple tissues from freshwater and marine subspecies,as well as tissues preserved using different solutions such as ethanol and formalin were collected for SIA.Linear mixed effects models were used for data analysis.Our results showed that,except for blubber,kidney,and stomach,differences between other tissues were correctable.In tissues from live sampling,we found no significant difference between blood and muscle,and skin could also be used for isotope analysis after proper correction.Ethanol preservation caused significant positive changes in δ^(13)C and δ^(15)N values of muscle,while formalin preservation caused negative changes in δ^(13)C and δ^(15)N.Our findings provide valuable insight into unifying data from stranded carcasses and live sampling,as well as correcting for the effect of chemical preservation on museum specimens.Findings from this research support further application of stable isotope analysis in the conservation of endangered finless porpoises,offer a reference for other similar cetaceans,and also provide guidance for chemical preservation when freezing conditions are not available.

关 键 词：Isotope ecology Linear regression Linear mixed effects model Tissue isotope values Preservation effect 

分 类 号：Q178.1[生物学—水生生物学]