9种重楼属植物叶中甾体皂苷成分差异的研究  

Research on the Differences of the Active Ingredients of Steroid Saponins in the Leaves of Paris L.

作　　者：李龙星 李海峰 王成军 LI Longxing;LI Haifeng;WANG Chengjun(Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from West Yunnan(Cultivation),Dali 671000,China;College of Pharmacy,Dali University,Dali 671000,China;Analysis and Testing Center,Dali University,Dali 671000,China)

机构地区：[1]云南省滇西抗病原植物资源筛选研究重点实验室(培育),云南大理671000 [2]大理大学药学院,云南大理671000 [3]大理大学分析测试中心,云南大理671000

出　　处：《中国民族民间医药》2023年第19期12-18,29,共8页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：云南省地方本科高校基础研究联合专项-面上项目(2019FH001-031)。

摘　　要：目的:探讨9种重楼属植物叶中甾体皂苷有效成分和主成分的差异。方法:采用液质联用(LC-MS)技术定性分析9种重楼属植物叶中甾体皂苷有效成分和主成分的差异及相关性。结果:金线重楼、南重楼、七叶一枝花叶中均检测到重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅲ(薯蓣皂苷)、重楼皂苷Ⅴ、重楼皂苷Ⅵ、重楼皂苷Ⅶ、重楼皂苷H、纤细薯蓣皂苷8种甾体皂苷有效成分,黑籽重楼、多叶重楼、滇重楼(阔瓣)叶中检测到7种甾体皂苷有效成分,滇重楼、毛重楼叶中检测到6种甾体皂苷有效成分,长柱重楼叶中检测到4种甾体皂苷有效成分,其中重楼皂苷Ⅰ、纤细薯蓣皂苷是重楼属植物叶中的主要存在形式;根据重楼属植物叶正离子模式下液质数据的主成分分析(PCA)模型可将长柱重楼、金线重楼、滇重楼、多叶重楼、南重楼、七叶一枝花聚为一类,其主要成分无显著差异。结论:金线重楼、南重楼、七叶一枝花叶中8种主要甾体皂苷有效成分和化学成分种类无显著差异。Objective To investigate the differences and correlation of the active ingredients of steroid saponins and principal component in the leaves of Paris L..Methods The differences and correlation of the active ingredients of steroid saponins and principal component in the leaves of Paris L.were qualitative analysis by LC-MS.Results Detect the active ingredients of PolyphyllinⅠ,PolyphyllinⅡ,PolyphyllinⅢ(Dioscin),PolyphyllinⅤ,PolyphyllinⅥ,PolyphyllinⅦ,Polyphyllin H and Gracillinin the leaves of P.delavayi,P.vietnamensis and var.chinensis,thereare sevenactive ingredientsin the leaves of P.thibetica,P.polyphylla and var.yunnanensis-1and six active ingredients in the leaves of P.mairei and var.yunnanensis,but only four active ingredients in the leaves of P.forrestii,PolyphyllinⅠand Gracillin was the main form of steroid saponins in the leaves of Paris L.;P.delavayi,P.vietnamensis,P.forrestii,P.polyphylla,var.chinensis and var.yunnanensis can be classified into one categories according to the PCA model of the data of LC-MS under the positive ion mode of Paris L..Conclusion There is little differences of active ingredients of steroid saponins and chemical composition in the leaves of P.delavayi,P.vietnamensis and var.chinensis.

关 键 词：重楼属 叶 液质联用 甾体皂苷 

分 类 号：R284.1[医药卫生—中药学]