基于网络药理学探讨青楷槭的抗弓形虫作用机制  

Study on the mechanism of anti-Toxoplasma gondii action of Acer tegmentosum Maxim based on network pharmacology

作　　者：屈金燕 王珈亿 杜鹏 于洪鹏 全星雨 张昌浩[1] QU Jinyan;WANG Jiayi;DU Peng;YU Hongpeng;QUAN Xingyu;ZHANG Changhao(Yanbian University College of Pharmacy,Yanji 133002,Jilin,China)

机构地区：[1]延边大学药学院,吉林延吉133002

出　　处：《延边大学医学学报》2023年第2期87-94,共8页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金项目(编号:82160741)。

摘　　要：[目的]应用网络药理学方法探究青楷槭抗弓形虫病作用的可能机制.[方法]通过检索Swiss Target Prediction数据库得到青楷槭的成分和靶点、检索Disgenent数据库获得弓形虫相关靶点,取两者相关靶点的交集为青楷槭抗弓形虫的活性成分及作用靶点,使用Cytoscape 3.7.2软件构建青楷槭活性成分-抗弓形虫病作用靶点网络并进行关键化合物的筛选,利用STRING平台建立靶蛋白的相互作用网络并筛选关键靶点,再通过R 3.6.1软件对靶点基因进行GO富集分析和KEGG代谢通路分析,明确生物学机制.[结果]共检索出青楷槭中的33个主要活性成分、446个相关靶基因和876个疾病靶点,两者取交集得到66个青楷槭-弓形虫病共同靶点.药物-成分-靶点-疾病网络与靶蛋白相互作用网络分析结果显示,TgHSP70是青楷槭抗弓形虫的关键靶点.[结论]青楷槭中的多个成分通过作用于多个靶点和通路发挥抗弓形虫作用.OBJECTIVE To explore the possible mechanism of anti-toxoplasmosis of Acer tegmentosum Maxim based on network pharmacology.METHODS The components and targets of Acer tegmentosum were obtained by retrieving Swiss target prediction database,and Toxoplama gondii related targets were obtained by searching Disgenent database.The intersection of the two related targets was selected as the active components and targets of Acer tegmentosum against Toxoplama gondii.Cytoscape 3.7.2 software was used to construct the network of active components of Acer tegmentosum and anti-toxoplasmosis targets,and screen the key compounds.The interaction network of target proteins was established with STRING platform and the key targets were screened,then R 3.6.1 software was used to conduct GO enrichment analysis and KEGG metabolic pathway analysis of target genes to clarify the biological mechanism.RESULTS A total of 33 main active components,446related-targets genes and 876 disease targets in Acer tegmentosum were retrieved,and 66 common targets of Acer tegmentosum and toxoplasmosis were obtained by intersection of the two.The results of a comprehensive analysis of the drug-component-target-disease network and target protein interaction network showed that TgHSP70 was the key target of Acer tegmentosum against Toxoplama gondii.CONCLUSION Several components of Acer tegmentosum play the anti-Toxoplama gondii role by acting on multiple targets and pathways.

关 键 词：青楷槭 弓形虫 网络药理学 

分 类 号：R285[医药卫生—中药学]