钴−生物质炭人工酶的制备及其在土壤氯辛硫磷残留检测中的应用  

Synthesis of Cobalt-biocarbon Mimic Enzyme and its Application in Detecting Chlorphoxim in Soil

作　　者：曹志军 贺根和[1] 张凯 罗旭彪 CAO Zhi-jun;HE Gen-he;ZHANG Kai;LUO Xu-biao(School of Life Sciences,Jinggangshan University,Jiangxi Ji’an 343009,China)

机构地区：[1]井冈山大学生命科学学院,江西吉安343009

出　　处：《南昌航空大学学报（自然科学版）》2023年第3期95-102,共8页Journal of Nanchang Hangkong University(Natural Sciences)

基　　金：江西省自然科学基金项目(20202ACBL203011)。

摘　　要：金属纳米材料人工酶由于具有较高的类氧化酶活性、良好的稳定性和较低的制备成本而被广泛研究和应用于生物传感与催化领域。本研究将梨瓜皮溶解分散于钴基水杨酸咪唑离子液体得到均匀的浆液,冷冻干燥后,经高温热解制得钴−生物质炭纳米复合材料人工酶。研究表明,钴−生物质炭纳米复合材料具有优异的分散性,平均粒径为50 nm,钴纳米粒子分布于生物质炭片表面,使其展现出较高的类氧化酶活性。基于氯辛硫磷对钴−生物质炭人工酶活性的抑制作用,建立测定氯辛硫磷的比色检测方法。氯辛硫磷的存在将抑制钴−生物质炭人工酶催化3,3’,5,5’-四甲基联苯胺(TMB)氧化成蓝色产物的活性,使得TMB氧化产物体系吸光度下降。反应体系的吸光度随着氯辛硫磷浓度(0.5~50μmol•L^(−1))的增大而线性降低,检出限为40 nmol•L^(−1)。这一检测方法简便快捷,可用于土壤中氯辛硫磷残留的快速测定。Metallic nanomaterial artificial enzymes have been studied and applied in the field of biocatalysis and biosensing due to their high peroxidase-like activity,stability,and low preparation cost.In this study,melon peel was dissolved and dispersed in a imidazolium Co-based salicylate ionic liquid to obtain a homogeneous slurry.Cobalt-biomass carbon nanocomposite artificial enzymes were synthesized after the high-temperature pyrolysis of the resultant solid from the above slurry by freeze-drying.The results show that the cobalt-biomass carbon nanocomposites(ca.50 nm in diameter)has excellent dispersibility and distribute on the surface of biomass carbon sheets,which exhibit high peroxidase-like activity.Based on the inhibitory effect of chlorphoxim on the activity of cobalt-biomass carbon artificial enzymes,a colorimetric detection method for chlorphoxim was proposed.As chlorphoxim can inhibit the activity of cobalt-biomass carbon artificial enzymes in catalyzing the oxidation of 3,3’,5,5’-Tetramethylbenzidine(TMB),the yield of corresponding blue product is declined,presented as decreased absorbance.The absorbance of the reaction system decreases linearly with the increased chlorphoxim concentration when ranging from 0.5 to 50μmol•L^(−1),and the detection limit is 40 nmol•L^(−1).The detection method is simple and rapid,which can be used for the fast determination of chlorphoxim residues in soil.

关 键 词：生物质炭 农业土壤 类氧化酶 农药残留 比色检测 

分 类 号：O657.3[理学—分析化学] TS255.7[理学—化学]