舒筋健脑方对缺血缺氧脑瘫模型大鼠细胞凋亡的影响  

Effects of Shujin Jiannao Prescription on cell apoptosis in rats with hypoxic-ischemic brain injury

作　　者：姜煜 徐林[1] 赵亚林 刘港 张亚奇 白惠中 任敬佩 曾杰 穆晓红[1] Jiang Yu;Xu Lin;Zhao Yalin;Liu Gang;Zhang Yaqi;Bai Huizhong;Ren Jingpei;Zeng Jie;Mu Xiaohong(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100007,China;Fengtai Rehabilitation Hospital of Beijing Municipality,Beijing 100161,China;Chongqing Traditional Chinese Medicine Hospital,Chongqing 400011,China)

机构地区：[1]北京中医药大学东直门医院,北京市100007 [2]北京市丰台康复医院,北京市100161 [3]重庆市中医院,重庆市400011

出　　处：《中国组织工程研究》2024年第28期4477-4483,共7页Chinese Journal of Tissue Engineering Research

基　　金：北京市自然科学基金面上项目(7222281),项目负责人:穆晓红。

摘　　要：背景:围产期缺血缺氧性脑损伤是脑性瘫痪发生的最常见原因之一。舒筋健脑方是北京中医药大学东直门医院骨科治疗脑瘫、改善脑部供血的经验方剂。目的:探讨舒筋健脑方改善脑性瘫痪缺血缺氧的作用机制。方法:采用64只新生7 d龄SD大鼠,随机分为6组,对照组、模型组各12只,米诺环素组、舒筋健脑方低、中、高剂量组各10只。除对照组外,其余各组建立新生大鼠缺血缺氧脑瘫模型。造模成功后的药物组大鼠分别进行米诺环素、舒筋健脑方各剂量[4,8,16 g/(kg·d)]灌胃,每日1次,持续1周。给药前后测量各组大鼠的体质量,检测行为学变化,苏木精-伊红染色观察大鼠脑组织海马CA1区组织形态,免疫组化和Western Blot检测大鼠脑组织Bcl-2、Bax、Caspase-3的表达水平。结果与结论:①体质量:与模型组比较,舒筋健脑方中、高剂量组大鼠体质量得到显著增加(P<0.05);②行为学:与模型组比较,米诺环素组大鼠有效延长了悬吊时间(P<0.05),舒筋健脑方中、高剂量组大鼠有效延长了悬吊时间、缩短了倾斜板实验时间、提升了Longa评分(P<0.05);③组织形态:药物组大鼠脑组织仅少量神经细胞坏死形成,细胞排列较整齐,细胞结构较为完整,只部分细胞核变小;④免疫组化和Western Blot结果:与模型组比较,米诺环素组、舒筋健脑方中、高剂量组Bax和Caspase-3的表达减少(P<0.05),舒筋健脑方中、高剂量组Bcl-2表达增加(P<0.05),米诺环素组、舒筋健脑方各剂量组Bcl-2/Bax蛋白表达增加(P<0.05),蛋白表达量随舒筋健脑方的剂量增加而增加,且舒筋健脑方高剂量组与米诺环素组作用差异无显著性意义(P>0.05);⑤结果说明,舒筋健脑方治疗缺血缺氧脑瘫大鼠的机制可能是提升抗凋亡蛋白Bcl-2表达,抑制促凋亡蛋白Bax的表达,降低Caspase-3的蛋白表达,最终抑制脑瘫大鼠海马组织细胞凋亡;舒筋健脑方在一定范围内,剂量越�BACKGROUND:Perinatal hypoxic-ischemic brain injury is one of the most common causes of cerebral palsy.Shujin Jiannao Prescription is an experienced formula for treating cerebral palsy and improving blood supply to the brain developed by the Dongzhimen Hospital,Beijing University of Chinese Medicine.OBJECTIVE:To explore the possible mechanism of Shujin Jiannao Prescription in treating hypoxic-ischemic cerebral palsy.METHODS:Sixty-four 7-day-old Sprague-Dawley rats were randomly divided into six groups.There were 12 rats in each of the control and model groups as well as 10 animals in each of the minocycline group,and the low-,medium-,and high-dose groups of Shujin Jiannao Prescription.The neonatal rat ischemic-hypoxic cerebral palsy model was established in all groups except for the control group.After successful modeling,rats in each drug group were respectively gavaged with minocycline and Shujin Jiannao Prescription at a dose of 4,8,and 16 g/kg per day for 1 week.Body mass of rats was measured and behavioral changes were detected before and after drug administration.Hematoxylin-eosin staining was used to observe the histomorphology of hippocampal CA1 region of rat brain tissue,and immunohistochemistry and western blot were used to detect the expression levels of Bcl-2,Bax,and Caspase-3 in the brain tissue of rats.RESULTS AND CONCLUSION:Compared with the model group,medium-and high-dose Shujin Jiannao Prescription significantly increased the body mass of rats(P<0.05).Compared with the model group,minocycline effectively prolonged the suspension time of ischemic-hypoxic cerebral palsy rats(P<0.05),while medium-and high-dose Shujin Jiannao Prescription significantly prolonged the suspension time,shortened the inclined plane test time,and increased the Longa score of rats(P<0.05).The pathological results showed that after drug intervention,only a small number of neuronal cells in the brain tissue of rats were necrotic,the cells were more neatly arranged,the cell structure was more complete,and only part of the cell

关 键 词：脑性瘫痪 舒筋健脑方 细胞凋亡 缺血缺氧脑损伤 大鼠 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R446