Evaluation of the famous classic formula Sanhua decoction based on network pharmacology and multi-component quantitative analysis  

作　　者：Xin Zhang Wan-Cui Wang Jin-Kui Zhang Wei-Mei Zhang Peng-Wang Wang Peng-Cheng Lin Yong-Chang Lu Xia Li Wen-Yuan Gao 

机构地区：[1]College of Pharmacy,Qinghai Minzu University,Xining 810000,China [2]Qinghai Key Laboratory of Phytochemistry on Qinghai-Tibet Plateau,Qinghai Minzu University,Xining 810000,China [3]School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300000,China [4]Qinghai Engineering Technology Research Center of Modern Tibetan Medicine Creation,Qinghai Minzu University,Xining 810000,China

出　　处：《Traditional Medicine Research》2024年第1期1-13,共13页TMR传统医学研究

基　　金：supported by grants from the Special Project for Transformation of Scientific and Technological Achievements in Qinghai Province(No.2021-SF-150);the National Natural Science Foundation of China(No.82173929).

摘　　要：Background:Sanhua decoction has significant effects in the treatment of stroke.The study of the Sanhua decoction material benchmark was carried out to analyze the value transfer relationship between the Chinese herbal pieces and the substance benchmark.Methods:Network pharmacology was employed to investigate the potential active components and molecular mechanisms of Sanhua decoction in the treatment of stroke.15 batches of Sanhua decoction lyophilized powder were prepared using traditional formulas and subjected to high-performance liquid chromatography analysis to generate fingerprints of the Sanhua decoction substance benchmarks.Then,a multi-component quantitative analysis method was established,allowing for the simultaneous determination of ten components,to study the transfer of quantity values between pieces and substance benchmarks.Results:60 active ingredients were screened from Sanhua decoction by network pharmacology,of which gallic acid,magnolol honokiol,physcion,and aloe-emodin may have a greater effect than other active components.63 key targets and 134 pathways were predicted as the potential mechanism of Sanhua decoction in treating stroke.The fingerprint similarity of the Sanhua decoction substance benchmarks was found to be good among the 15 batches,confirming the 19 common peaks.The content of the 10 components was basically consistent.The components’transfer rates were within 30%of their respective means.Conclusions:This study provided a comprehensive and reliable strategy for the quality evaluation of Sanhua decoction substance benchmarks and held significant importance in improving its application value.

关 键 词：Sanhua decoction classic famous formula HPLC fingerprinting value transfer network pharmacology 

分 类 号：R285[医药卫生—中药学]