螟黄赤眼蜂携带球孢白僵菌防治大豆食心虫  

Control Effect of Trichogramma chilonis carrying Beauveria bassiana on Soybean Podborer

作　　者：赵宇[1] 刘佳[2] 路杨[1] 隋丽[1] 徐文静[1] 杜茜[1] 李启云[1] 张正坤[1] ZHAO Yu;LIU Jia;LU Yang;SUI Li;XU Wenjing;DU Qian;LI Qiyun;ZHANG Zhengkun(Institute of Plant Protection,Jilin Academy of Agricultural Science/Jilin Key Laboratory of Agricultural Microbiology/Key Laboratory of Integrated Pest Management on Crops in Northeast China,Ministry of Agriculture and Rural Affairs,Gongzhuling 136100,China;Institute of Soybean Research,Jilin Academy of Agricultural Sciences,Changchun 130033,China)

机构地区：[1]吉林省农业科学院植物保护研究所/吉林省农业微生物重点实验室/农业农村部东北作物有害生物综合治理重点实验室,公主岭136100 [2]吉林省农业科学院大豆研究所,长春130033

出　　处：《中国生物防治学报》2023年第4期760-766,共7页Chinese Journal of Biological Control

基　　金：吉林省科技发展计划重点研发项目(20210202117NC);吉林省创新创业人才资助项目(2021Y016)。

摘　　要：大豆食心虫作为重要害虫在我国大豆主产区造成巨大经济损失。白僵菌和赤眼蜂是大豆食心虫的两种重要生物防治手段,如何将二者相结合实现大豆食心虫的可持续防控还未见报道。本研究从大豆食心虫幼虫僵虫分离、鉴定并筛选到3株高毒力球孢白僵菌菌株,对大豆食心虫老熟幼虫致死中时间(LT50)分别为6.21 d,6.39 d和6.87 d。筛选到对螟黄赤眼蜂羽化和寄生害虫卵无明显影响的球孢白僵菌孢子高吸附性助剂0.1%(w/v)淀粉溶液,实现单头蜂带菌量3.56×10^(4)个孢子。两年田间试验表明,较单一使用螟黄赤眼蜂,螟黄赤眼蜂携带球孢白僵菌对大豆食心虫防治效果分别提高了11.80%和8.31%,分别达到了70.44%和64.75%,从而实现了在不明显增加防治成本的基础上,提高防治效率,实现协同增效。Soybean moth is an important pest causing huge economic loss in the main soybean producing areas in China.Beauveria bassiana and Trichogramma are the two important biological control agents against soybean moth.However,how to combine the two agents to realize sustainable control of soybean moth is not addressed.Herein,three B.bassiana strains with high virulence against the pest were isolated,identified and screened from the diseased soybean moth larvae.The median lethal time(LT50)of the strains to the mature larvae of the pest were 6.21 days,6.39 days and 6.87 days,respectively.The B.bassiana conidia and their highly adsorptive adjunct,0.1%(w/v)starch solution,showed no effect on the emergence and parasitism of pest eggs of T.chilonis.With the adjunct,T.chilonis was able to carry fungal conidia of 3.56×10^(4) per wasp.The two-year field experiments showed that,compared with Trichogramma alone,the pest control effect of Trichogramma carrying B.bassiana was increased by 11.80%and 8.31%,respectively,to 70.44%and 64.75%,respectively,thereby improving the pest control efficiency and realizing synergy without increasing the control cost.

关 键 词：大豆食心虫 生防微生物 天敌 协同增效 

分 类 号：S476[农业科学—农业昆虫与害虫防治]