短短芽胞杆菌B011基因组中芽胞形成调控基因spo0E的鉴定与表达分析  

Identification and Expression Analysis of spo0E Gene Family in Brevibacillus brevis Strain B011

作　　者：周向平[1,2] 王运生 陈武[1] 刘天波[3] 王凯歌 刘峰 周立 袁志辉 ZHOU Xiangping;WANG Yunsheng;CHEN Wu;LIU Tianbo;WANG Kaige;LIU Feng;ZHOU Li;YUAN Zhihui(Plant Protection College,Hunan Agricultural University,Changsha 410128,China;Yongzhou Company of Hunan Tobacco Company,Yongzhou 425100,China;Hunan institute of Tobacco Science,Changsha 410004,China;College of Chemistry and Bioengineering,Hunan University of Science and Engineering,Yongzhou 425199,China;Hunan Provincial Engineering Research Center for Ginkgo Biloba,Yongzhou 425199,China)

机构地区：[1]湖南农业大学植物保护学院,长沙410128 [2]湖南省烟草公司永州市公司,永州425100 [3]湖南省烟草科学研究所,长沙410004 [4]湖南科技学院化学与生物工程学院,永州425199 [5]湖南省银杏工程技术研究中心,永州425199

出　　处：《中国生物防治学报》2023年第4期895-903,共9页Chinese Journal of Biological Control

基　　金：国家重点研发计划支持项目(2021YFD1400805);湖南省烟草公司永州市公司科技项目(YZ2022KJ04);湖南省教育厅科学研究项目重点项目(21A0519);湖南省自然科学基金项目(2023JJ50070,2022JJ30272)。

摘　　要：短短芽胞杆菌B011是一株具有广谱抗菌活性的植物内生菌,发酵后期形成芽胞。据报道,芽胞形成调控基因Spo0E的编码产物能对Spo0A~P蛋白因子去磷酸化,从而实现芽胞形成的负调控。为了能够进一步验证B011基因组中Spo0E基因的功能和表达情况,为B011菌株的遗传改造提供候选基因,本研究基于B011全基因组序列,通过生物信息学工具预测得到了9个spo0E类似基因,并与其他已公布全基因组序列的101个短芽胞杆菌Brevibacillus菌株进行比较,结果表明短短芽胞杆菌spo0E家族基因在物种分化前就已经存在。转录组测序结果表明,FO446_19435和FO446_04050两个spo0E家族基因在B011中高表达,表达模式表现为先升高,在发酵18 h达到最高,此后下降,这两个基因均存在SQELD基序,推测这两个基因为B011中的两个主效spo0E基因。研究结果可为短短芽胞杆菌B011的进一步开发和利用提供一定的基础。Brevibacillus brevis B011 is an endophytic bacteria,which was isolated from the healthy tobacco root,and could produce endospores at the late stages of fermentation.According to reported results,the product of the Spo0E gene can dephosphorylate Spo0A~P and then regulates spore formation negatively.In order to further verify the function and expression of the Spo0E gene in the B011 genome and provide candidate genes for genetic modification of the strain,we identified 9 spo0E-like genes in the whole genome sequences of strain B011 using bioinformatics tools.The spo0E gene families in Brevibacillus were differentiated before the separation of species by comparing the whole genome of 101 strains of Brevibacillus.Two spo0E-like genes,FO446_19435 and FO446_04050,were abundantly expressed in B011 and both of them increased first,then decreased and the expression level reached the highest peak in the broth after 18 h.Both of these two spo0E-like genes contained SQELD motif and we proposed they are the two main spo0E genes in strain B011.

关 键 词：短短芽胞杆菌 芽胞 全基因组 spo0E基因 

分 类 号：Q939.96[生物学—微生物学]