寨卡病毒核酸检测技术研究进展  

Advance in research on the nucleic acid detection of Zika virus

作　　者：张智慧 王志江 彭沫溱 李茜 苏品璨[1] ZHANG Zhihui;WANG Zhijiang;PENG Mozhen;LI Qian;SU Pincan(Yunnan Kunming Blood Center,Kunming,Yunnan,650106,China)

机构地区：[1]云南昆明血液中心,云南昆明650106

出　　处：《当代医学》2023年第23期187-190,共4页Contemporary Medicine

基　　金：云南省第一人民医院临床医学中心分中心开放项目(2021LCZXXF-XY16);血液筛查技术科研课题(XS2020-4)。

摘　　要：寨卡病毒(ZIKV)为黄病毒科黄病毒属的一种单链RNA病毒,主要通过埃及伊蚊和白纹伊蚊传播,也可通过血液、性、母婴等途径传播。传播ZIKV的蚊虫在我国分布广泛,同时,随着全球化的发展,世界各国人员往来日益频繁,ZIKV在我国有潜在传播的可能性。核酸检测技术是实验室快速检测的有效手段,对预防和控制ZIKV传播具有重要意义。本文介绍了ZIKV基因组结构,并对ZIKV核酸检测的样本特点及方法进展作一综述。Zika virus(ZIKV)belongs to the genus Flavivirus of the family Flaviviridae and is a single-stranded RNA virus,mainly transmitted by Aedes aegypti and Aedes albopictus,it can also be transmitted through blood transfusions,sex,mother-to-child and so on.The kind of mosquito that transmits ZIKV does widely exist in China,at the same time,with the development of globalization and the increasingly frequent exchanges of people from all over the world,the ZIKV has the potential to spread in China.Nucleic acid detection technology is an effective means of rapid detection in laboratory,which is of great significance for prevention and control the transmission of ZIKV.In this paper,the genomic structure of ZIKV is introduced,and the characteristics of samples and detection methods for ZIKV nucleic acid detection are reviewed.

关 键 词：寨卡病毒 核酸检测 逆转录-聚合酶链反应 逆转录-环介导等温扩增技术 逆转录重组酶聚合酶扩增技术 

分 类 号：R373[医药卫生—病原生物学]