基于ICP-MS及多元统计分析相结合的地黄酵素发酵前后无机元素特征  

Characteristics of Inorganic Elements before and after Fermentation of Rehmannia Glutinosa Based on ICP-MS and Multivariate Statistical Analysis

作　　者：何江龙 裴莉昕[1] 张童童 纪宝玉[1] 娄玉霞[1] 陈随清[1] 董诚明[1] HE Jianglong;PEI Lixin;ZHANG Tongtong;JI Baoyu;LOU Yuxia;CHEN Suiqing;DONG Chengming(College of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou,Henan 450046,China)

机构地区：[1]河南中医药大学药学院,郑州450046

出　　处：《中国无机分析化学》2023年第11期1255-1263,共9页Chinese Journal of Inorganic Analytical Chemistry

基　　金：河南省中医药科学研究专项项目(20-21ZY2143);国家级大学生创新创业训练计划项目(202110471001);国家重点研发计划项目(2017YFC1702800)。

摘　　要：探讨地黄酵素制备前后无机元素的变化,分析发酵对地黄中无机元素的影响,为地黄酵素的制备开发提供数据支撑和理论依据。以四批不同来源的地黄为原料,利用乳酸菌和酵母菌发酵制备地黄酵素,采用微波消解-电感耦合等离子质谱(ICP-MS)法结合主成分分析、差异性分析和Pearson相关性分析等多元统计学方法对地黄酵素发酵前后样品中24种无机元素含量变化特征进行研究。结果表明,发酵对地黄原料中无机元素的种类没有影响,但对无机元素的含量影响较大,发酵前后24种无机元素的分类特征明显不同,其中发酵前后Na、P、Mn、Se、Sr和Cd 6种元素的含量差异在统计学上达到显著水平,而且地黄酵素发酵过程中各无机元素之间的代谢相互影响,其中Ca和Ti之间存在极显著的正相关关系,Pearson相关系数高达0.97;P和Mn,Ba和Pb存在显著的负相关关系,Pearson相关系数在-0.80以下。采用所建立的电感耦合等离子体质谱法测定中草药保健食品地黄酵素中的无机元素,并对地黄发酵制备地黄酵素前后的无机元素特征进行分析,可为地黄酵素在功能性食品添加剂方面的研究和地黄酵素的制备开发提供数据支撑,测定结果稳定可靠,能满足地黄酵素中24种无机元素含量的分析检测要求,也可为其他中草药功能性保健食品中无机元素的检测提供方法参考。To explore the changes of inorganic elements before and after the preparation of Rehmannia glutinosa,and analyze the effect of fermentation on inorganic elements in Rehmannia glutinosa,so as to provide data support and theoretical basis for the preparation and development of Rehmannia glutinosa.Four batches of Rehmannia glutinosa from different sources were used as raw materials,and lactic acid bacteria and yeast were used to ferment Rehmannia glutinosa.Microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS)was used to combine principal component analysis,difference analysis and Pearson correlation.Analysis and other multivariate statistical methods were used to study the content change characteristics of 24 kinds of inorganic elements in samples before and after fermentation of Rehmannia glutinosa.It was found that fermentation had no effect on the types of inorganic elements in the raw materials of Rehmannia glutinosa,but had a greater impact on the content of inorganic elements.The classification characteristics of the 24 inorganic elements before and after fermentation were significantly different,among which Na,P,Mn,Se,Sr and Cd reached a statistically significant level,and the metabolic interaction between the various inorganic elements in the fermentation process of Rehmannia glutinosa,among which there was a very significant positive correlation between Ca and Ti elements,Pearson correlation the coefficient is as high as 0.97.There is a significant negative correlation between P and Mn,Ba and Pb,and the Pearson correlation coefficient is below-0.80.The established inductively coupled plasma mass spectrometry was used to determine the inorganic elements in Rehmannia enzymes of Chinese herbal health food,and the characteristics of inorganic elements before and after fermentation of Rehmannia glutinosa were analyzed,which could provide data support for the research of Rehmannia enzymes in functional food additives and the preparation and development of Rehmannia enzymes.The determination res

关 键 词：地黄酵素 ICP-MS 无机元素 差异分析 相关性分析 

分 类 号：O657.63[理学—分析化学] R28[理学—化学]