微囊藻毒素检测用电化学生物传感器的研究  

Study on Electrochemical Biosensors for Microcystin Detection

作　　者：古瑞琴 付会兵 黄清[1] 赵雨农 刘欢[1] GU Ruiqin;FU Huibing;HUANG Qing;ZHAO Yunong;LIU Huan(School of Integrated Circuits,Huazhong University of Science and Technology,Wuhan National Laboratory for Optoelectronics,Wuhan Optics Valley Laboratory,Wuhan 430074,China;Zhengzhou Winsen Electronic Technology Co.,LTD.,Zhengzhou 450001,China)

机构地区：[1]华中科技大学集成电路学院,武汉光电国家研究中心,湖北光谷实验室,湖北武汉430074 [2]郑州炜盛电子科技有限公司,河南郑州450001

出　　处：《仪表技术与传感器》2023年第9期119-126,共8页Instrument Technique and Sensor

基　　金：湖北省自然科学基金项目(2022CFA035)。

摘　　要：微囊藻对人体肝脏等器官具有较强的毒性。对微囊藻毒素的高灵敏、快速检测有助于保障饮用水的安全。文中利用二氧化锡胶体量子线修饰金电极,进一步包被微囊藻抗体,构建出一种对微囊藻毒素具有高灵敏度和特异性的电化学生物传感器。差分脉冲伏安法测试结果表明:在1 pg/mL~10 ng/mL微囊藻毒素质量浓度范围内,传感器响应具有较好的线性,检测下限为0.33 pg/mL。进一步的研究表明:二氧化锡修饰提高了电极表面对微囊藻抗体的富集能力,同时具有促进电荷传输的作用。Microcystis has strong toxicity to human liver and other organs.The highly sensitive and rapid detection of microcystin is helpful to ensure the safety of drinking water.In this paper,an electrochemical biosensor with high sensitivity and specificity to microcystin was constructed,in which the gold electrode was modified with tin oxide colloidal quantum wires and coated with microcystin antibody.The measurement results using differential pulse voltammetry method indicates that the sensor response has a good linearity toward microcystin in the concentration range of 1 pg/mL~10 ng/mL,and the limit of detection was 0.33 pg/mL.Further studies show that the tin oxide modification can improve the enrichment ability of microcystin antibody on the electrode surface and promote the charge transport ability.

关 键 词：微囊藻毒素 电化学生物传感器 二氧化锡 

分 类 号：TQ150[化学工程—电化学工业]