151份小麦品种(系)中主要品质基因的分子检测  

Molecule Detection of Main Quality Genes in 151 Wheat Lines

作　　者：张红 王歆 钱禄巧 金彦刚 杨永乐 夏中华 ZHANG Hong;WANG Xin;QIAN Luqiao;JIN Yangang;YANG Yongle;XIA Zhonghua(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,Anhui,China;Jiangsu Ruihua Agricultural Science&Technology Co.,Ltd./Jiangsu Wheat Commercial Improvement Engineering Technology Research Center,Suqian 223800,Jiangsu,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100 [2]江苏瑞华农业科技有限公司/江苏省小麦商业化育种工程技术研究中心,江苏宿迁223800

出　　处：《江汉大学学报（自然科学版）》2023年第5期14-23,共10页Journal of Jianghan University：Natural Science Edition

基　　金：江苏省重点研发项目(BE2021375)。

摘　　要：明确江苏瑞华农业科技有限公司151份小麦品种(系)中品质基因的分布情况,利用分子标记技术对小麦谷蛋白亚基(HMW-GS)、多酚氧化酶(PPO)活性和1B/1R异位系进行检测。结果表明:高分子量谷蛋白亚基(HMW-GS)基因频率Ax2为1.32%,Dx5为17.22%,By8为70.20%,Bx7为89.40%,Ax2+Dx5、Ax2+By8和Ax2+Bx7均为0.66%,Dx5+By8为7.28%,Dx5+Bx7为14.57%,By8+Bx7为62.25%;多酚氧化酶(PPO)活性相关基因频率Ppo-A1b为45.70%,Ppo-D1a为25.83%,双低PPO活性等位基因Ppo-A1b+Ppo-D1a为优异等位基因,频率为8.60%;1B/1R异位系的分布频率为86.75%;同时携带低PPO活性等位变异组合,且为非1B/1R异位系的材料只有2份,频率为1.32%;同时携带谷蛋白亚基Bx7和低PPO活性等位变异组合,且为非1B/1R异位系的材料只有1份,可作为小麦育种材料。To clarify the distribution of quality genes in 151 wheat varieties(lines)of Jiangsu Ruihua Agricultural Science and Technology Co.,Ltd.,molecular markers were used to detect high molecular weight glutenin subunit(HMW-GS),PPO activity,and 1B/1R ectopic lines.The results showed that the gene frequencies of HMW-GS Ax2,Dx5,By8,and Bx7 were 1.32%,17.22%,70.20%and 89.40%,respectively.The gene frequencies of Ax2+Dx5,Ax2+By8,and Ax2+Bx7 were all 0.66%,and the gene frequencies of Dx5+By8,Dx5+Bx7,and By8+Bx7 were 7.28%,14.57%and 62.25%,respectively.The frequency of polyphenol oxidase(PPO)activity-related genes Ppo-A1b and Ppo-D1a was 45.70%and 25.83%,double low PPO activity allele Ppo-A1b+Ppo-D1a was an excellent allele,with a frequency of 8.60%.The distribution frequency of 1B/1R was 86.75%.Only 2 materials were carrying low PPO activity allelic variation combinations and non-1B/1R ectopic lines at the same time,with a frequency of 1.32%.Only one material was carrying an allelic variation combination of glutenin subunit Bx7 and low PPO activity at the same time,which was a non-1B/1R ectopic line,and it could be used as wheat breeding material.

关 键 词：小麦 高分子量麦谷蛋白 多酚氧化酶 1B/1R异位系 分子标记 

分 类 号：S512.1[农业科学—作物学]