葱白提取物对心肌梗死大鼠促血管新生作用及机制研究  

Study on Angiogenesis Effect and Mechanism of Fistular Onion Bulb on Myocardial Infarction in Rats

作　　者：罗雨佳 雷杰 祝炜[1] 段刚峰[1] LUO Yujia;LEI Jie;ZHU Wei;DUAN Gangfeng(Department of Cardiovascular Medicine,Wuhan Hospital of Integrated Traditional Chinese and Western Medicine,Wuhan 430022,Hubei,China)

机构地区：[1]武汉市中西医结合医院心血管内科,湖北武汉430022

出　　处：《江汉大学学报（自然科学版）》2023年第5期60-66,共7页Journal of Jianghan University：Natural Science Edition

基　　金：武汉市卫生计生委员会科研项目(WZ16D03);新疆维吾尔自治区自然科学基金项目(2021D01F22)。

摘　　要：目的探讨葱白提取物(fistular onion bulb,FOB)对促血管新生(angiogenesis)的作用及其机制。方法将SD大鼠,随机分成葱白提取物组、麝香保心丸组、模型组、假手术组,每组10只。在适应性喂养1周后,行左冠脉前降支结扎造模。CD34免疫组化染色观察心肌梗死边缘区微血管数量(microvessel quantity,MVQ)及微血管密度(microvessel density,MVD);ELISA法检测血清转化生长因子-β(transforming growth factor-β,TGF-β)含量;Western Blot法检测大鼠左室心肌组织丝裂原激活蛋白激酶(mitogen activated protein kinase,MEK)、细胞外信号调节激酶(extracellular singnal-regulated kinase,ERK)蛋白含量;RT-PCR法检测心肌梗死边缘组织MEK、ERK mRNA表达量。结果与模型组相比,葱白提取物组、麝香保心丸组在CD34染色后心肌梗死边缘区微血管计数大幅升高(P<0.01);葱白提取物组微血管计数低于麝香保心丸组(P<0.01)。与模型组相比,葱白提取物组、麝香保心丸组血清TGF-β均增加(P<0.01,P<0.05)。葱白提取物组及麝香保心丸组MEK、ERK蛋白表达量均增加,MEK、ERK mRNA扩增倍数显著升高(P<0.01);与麝香保心丸组相比,葱白提取物组ERK蛋白表达量、ERK mRNA扩增倍数均较低(P<0.01,P<0.05),MEK蛋白表达量无明显差异(P>0.05)。结论葱白提取物具有促进梗死心肌血管新生作用,增加心肌梗死边缘区新生血管数目,其机制可能与上调血清TGF-β水平及MEK/ERK信号通路表达有关。Objective To probe the function and mechanism of the fistular onion bulb(FOB)in promoting angiogenesis on myocardial infarction in rats.Methods Randomly dividing the rats into 4 groups(10 in each group):the FOB group,the Shexiang Baoxin pill group,the model group,and the sham operation group.After one week of adaptive feeding,the model of left anterior descending coronary artery ligation was performed.The quantity and density of microvascular in the margin of myocardial infarction were observed by CD34 immunohistochemical staining.Serum TGF-βcontent was detected by ELISA.The protein levels of MEK and ERK in the left ventricular myocardium of rats were detected by Western Blot.The mRNA expression levels of MEK and ERK in the edge of myocardial infarction were detected by RT-PCR.Results Compared with the model group,The MVD value of CD34 staining in The FOB group and the Shexiang Baoxin pill group was significantly increased(P<0.01).The MVD value in The FOB group was lower than that in the Shexiang Baoxin pill group(P<0.01).Compared with the model group,the level of serum TGF-βin the FOB group was significantly increased(P<0.01),and the level of TGF-βin the Shexiang Baoxin pill group was also increased(P<0.05).The protein expression levels of MEK and ERK were increased,and the amplification factor of MEK and ERK mRNA was significantly increased(P<0.01).Compared with the Shexiang Baoxin pill group,the ERK protein expression level,and the ERK mRNA amplification multiple were lower in the FOB group(P<0.01,P<0.05),and the MEK protein expression level had no significant difference(P>0.05).Conclusion The FOB can promote the angiogenesis of myocardial infarction and increase the number of neovascularization at the edge of myocardial infarction.The mechanism may be related to the up-regulation of serum TGF-βlevel and the expression of the MEK/ERK signaling pathway.

关 键 词：葱白提取物 心肌梗死 血管新生 TGF-Β MEK/ERK 

分 类 号：R542.220.5[医药卫生—心血管疾病]