肥胖/超重对哮喘发作期患儿Th1/Th2和Th17/Treg细胞因子的影响  

Influence of obesity/overweight on inflammatory factors of Th1/Th2 and Th17/Treg in children with asthma attacks

作　　者：丁肖媛 戴劲盛[1] 焦荣红[2] 马素丽 朱海峰 吴梦雅 车艳然 张蕾[1] DING Xiaoyuan;DAI Jinsheng;JIAO Ronghong;MA Suli;ZHU Haifeng;WU Mengya;CHE Yanran;ZHANG Lei(Department of Pediatrics,Shanghai Pudong New Area People's Hospital,Shanghai 201299,China;Department of Clinical Laboratory,Shanghai Pudong New Area People's Hospital,Shanghai 201299,China)

机构地区：[1]上海市浦东新区人民医院儿科,上海201299 [2]上海市浦东新区人民医院检验科,上海201299

出　　处：《检验医学》2023年第8期748-752,共5页Laboratory Medicine

基　　金：上海市卫生健康委员会青年科研项目(20204Y0110);上海市浦东新区卫生健康委员会优秀青年医学人才培养项目(PWRq2020-38)。

摘　　要：目的探讨肥胖/超重对哮喘发作期儿童外周血辅助性T细胞(Th)1炎症因子[肿瘤坏死因子α(TNF-α)]、Th2炎症因子[白细胞介素(IL)-10、IL-13]、Th17炎症因子(IL-17F)和调节性T细胞(Treg)炎症因子(IL-35)水平的影响。方法选取2020年1月—2021年12月上海市浦东新区人民医院哮喘患儿112例,根据体重指数(BMI)分为哮喘组(56例)、肥胖/超重合并哮喘组(56例),以肥胖/超重的非哮喘患儿56例(肥胖/超重组)作为对照。检测所有研究对象TNF-α、IL-10、IL-13、IL-17F、IL-35和IL-6、瘦素水平。采用Spearman相关分析评估BMI与各项指标之间的相关性。结果哮喘组、肥胖/超重合并哮喘组和肥胖/超重组之间血清IL-6、IL-10、IL-13和瘦素水平差异均有统计学意义(P<0.05)。肥胖/超重合并哮喘组血清TNF-α水平显著高于哮喘组(P<0.05)。肥胖/超重组血清IL-17F水平显著高于哮喘组和肥胖/超重合并哮喘组(P<0.05)。哮喘组血清IL-35水平显著高于肥胖/超重合并哮喘组和肥胖/超重组(P<0.05)。将肥胖/超重合并哮喘患儿和肥胖/超重非哮喘患儿进一步分为超重合并哮喘组、肥胖合并哮喘组、超重组和肥胖组。Spearman相关分析结果显示,超重合并哮喘组、肥胖合并哮喘组、超重组和肥胖组BMI与IL-6呈正相关(r值分别为0.515、0.510、0.515、0.432,P<0.05);超重合并哮喘组、肥胖合并哮喘组和肥胖组BMI与瘦素呈正相关(r值分别为0.488、0.578、0.546,P<0.05),与IL-35呈负相关(r值分别为-0.491、-0.517、-0.524,P<0.01)。结论肥胖/超重会引起Th1/Th2表达失衡,肥胖/超重合并哮喘患儿Th1炎症因子呈高表达。Objective To investigate the influence of obesity/overweight on T helper(Th)1 cell inflammatory factor[tumor necrosis factor-alpha(TNF-α)],Th2 cell inflammatory factors[interleukin(IL)-10,IL-13],Th17 cell inflammatory factor(IL-17F)and regulatory T cell(Treg)inflammatory factor(IL-35)at attack stage of asthma children in peripheral blood.Methods A total of 112 children with asthma at Shanghai Pudong New Area People's Hospital from January 2020 to December 2021 were enrolled and classified into asthma group(56 cases),obesity/overweight combined asthma group(56 cases)according to body mass index(BMI),and 56 non-asthma children with obesity/overweight(obesity/overweight group)were enrolled.The levels of TNF-α,IL-10,IL-13,IL-17F,IL-35,IL-6 and leptin in peripheral blood were determined.The correlation between BMI and indexes was evaluated by Spearman correlation.Results There was statistical significance in serum levels of IL-6,IL-10,IL-13 and leptin in asthma group,obesity/overweight combined asthma group and obesity/overweight group(P<0.05).The serum TNF-αlevel in obesity/overweight combined asthma group was higher than that in asthma group(P<0.05).The serum IL-17F level in obesity/overweight group was higher than those in asthma group and obesity/overweight combined asthma group(P<0.05).IL-35 in asthma group was higher than those in obesity/overweight combined asthma group and obesity/overweight group(P<0.05).The obesity/overweight combined asthma group and obesity/overweight group were further classified into overweight combined asthma group,obesity combined asthma group,overweight group and obesity group.BMI was positively correlated with IL-6 in overweight combined asthma group,obesity combined asthma group,overweight group and obesity group by Spearman correlation(r=0.515,0.510,0.515 and 0.432,P<0.05).BMI was positively correlated with leptin(r=0.488,0.578 and 0.546,P<0.05)and negatively correlated with IL-35(r=-0.491,-0.517 and-0.524,P<0.01).Conclusions Obesity/overweight can cause the imbalance of Th

关 键 词：肿瘤坏死因子α 白细胞介素10 白细胞介素13 白细胞介素17F 白细胞介素35 白细胞介素6 瘦素 辅助性T细胞 肥胖 哮喘 

分 类 号：R446.1[医药卫生—诊断学]