不同鸭品种脂肪型脂肪酸结合蛋白基因部分序列多态性差异研究  

Differences in polymorphism of some sequences of A-FABP gene in different duck breeds

作　　者：董飚[1] 王健[1,2] 孙国波 纪荣超[2] 张干生 DONG Biao;WANG Jian;SUN Guobo;JI Rongchao;ZHANG Gansheng(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;National Gene Bank of Waterfowl Resources,Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]国家级水禽基因库,江苏泰州225300

出　　处：《畜牧与兽医》2023年第10期1-5,共5页Animal Husbandry & Veterinary Medicine

基　　金：江苏高校“青蓝工程”项目(苏教师函[2021]11号)。

摘　　要：为了解地方鸭品种脂肪型脂肪酸结合蛋白(A-FABP)基因点突变信息、群体遗传结构和遗传参数差异,设计了1对引物扩增A-FABP基因外显子3和内含子2部分序列,采用PCR-SSCP方法检测9个地方鸭品种单核苷酸突变,并分析基因型、等位基因频率和群体遗传参数的差异。结果显示:在9个鸭品种之间发生了6个点突变,4个点突变在内含子2中,2个点突变在外显子3中,为同义突变。9个鸭品种均以D等位基因频率最高,E为靖西大麻鸭的第二等位基因,而其他8个鸭品种的第二等位基因为C。χ2检验显示靖西大麻鸭的基因型分布与巢湖鸭、临武鸭存在显著性差异(P<0.05),与其他品种存在极显著差异(P<0.01)。巢湖鸭的多态信息含量高于0.5,其他8个鸭品种的多态信息含量在0.25~0.5之间。攸县麻鸭偏离了哈代温伯格平衡状态。A-FABP基因在不同鸭品种中具有较丰富的多态性,为鸭品种资源保护和新品种培育提供参考。This study was to understand the mutation of the adipocyte fatty-acid bingding protein(A-FABP)gene,and the population ge⁃netic structure and genetic parameters of different domestic duck breeds.A pair of primers was designed to amplify the partial sequences of in⁃tron 2 and exon 3 of the A-FABP gene.The technique of PCR-SSCP was used to detect SNP of the A-FABP gene in 9 domestic duck breeds,and the differences in population genotype frequency,allele frequency and population genetic parameters were analyzed.The results were that six SNPs were found among the nine duck breeds,four SNPs being in intron 2 and two SNPs in exon 3;and the sequence of the amino acid didnt change.The frequency of the D allele was the highest in the 9 duck breeds.E was the second allele of Jingxi Large Par⁃tridge ducks,and C was the second allele of the other duck breeds.The χ^(2) test showed that the genotype distribution of Jingxi Large Partridge ducks was significantly different from that of Chaohu ducks and Linwu ducks(P<0.05),and very significantly different from that of the other breeds(P<0.01).The PIC of Chaohu ducks was higher than 05,and that of the other duck breeds were between 0.25 and 0.5.Youxian Partridge ducks deviated from the Hardy-Weinberg equilibrium.Different duck breeds exhibited affluent mononucleotide polymorphisms in the A-FABP gene,which would serve as reference for protection of duck breeds resources and new breed cultivation.

关 键 词：地方鸭品种 脂肪型脂肪酸结合蛋白 单核苷酸多态性 遗传分析 

分 类 号：S834[农业科学—畜牧学]