鸭甲型肝炎病毒3型对商品肉鸭的致病性研究  

Pathogenicity of duck hepatitis A virus 3 in commercial ducklings

作　　者：张永志 郝东敏 蓝胜芝 嵇康 马海营 邵红霞[1,4] 秦爱建 ZHANG Yonghzi;HAO Dongmin;LAN Shenzhi;JI Kang;MA Haiying;SHAO Hongxia;QIN Aijian(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Ceva Animal Health LLC,Hangzhou 310020,China;Anhui Qiangying Group,Dangshan 200040,China;Jiangsu Province Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]法国诗华动物保健公司,浙江杭州310020 [3]安徽强英鸭业集团,安徽砀山200040 [4]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《畜牧与兽医》2023年第10期37-41,共5页Animal Husbandry & Veterinary Medicine

基　　金：江苏高校优势学科建设工程资助项目。

摘　　要：为了解鸭甲型肝炎病毒3型(DHAV-3)对商品肉鸭的致病性,利用DHAV-3型强毒株SD20株滴口接种10日龄雏鸭,对攻毒后死亡雏鸭的组织病变进行了观察,并对攻毒后24和48 h雏鸭的血清生化指标进行了测定。结果显示:DHAV-3对雏鸭的致死率达85%,对肝脏等组织造成广泛的病理损伤,同时严重影响感染肉鸭的血清生化指标。攻毒后24 h,鸭血清中直接胆红素显著性降低(P<0.05),γ-谷氨酰转移酶和肌酐显著性升高(P<0.05);攻毒后48 h,鸭血清中白蛋白、直接胆红素显著升高(P<0.05),白球比、谷丙转氨酶、谷草转氨酶、γ-谷氨酰转移酶、尿素氮和肌酐均极显著升高(P<0.01),谷丙/谷草和血糖均极显著下降(P<0.01),而总蛋白、球蛋白、总胆红素、间接胆红素、碱性磷酸酶指标变化不显著(P>0.05)。研究结果提示,DHAV-3对商品肉鸭致病性较强,养殖过程中需要积极预防。This study was to observe the pathological changes and serum biochemical indexes changes in commercial ducklings infected with DHAV-3.10-day-old commercial ducklings were infected with DHAV-3 strain SD20 by oral route.The histopathological changes in the dead ducklings were observed,and the blood samples were collected and measured at 24 h and 48 h post infection.The results showed that the mortality rate of the ducklings infected with DHAV-3 strain SD20 was as high as 85%,and many tissues of the ducklings,such as liver tissues,exhibited extensive pathological damage;at the same time,many serum biochemical indicators changed significantly.The indexes of DBIL decreased significantly(P<0.05),but those of GGT and CR increased significantly(P<005),at 24 h post infection.However,at 48 h post infection,the indexes of ALB and DBIL increased significantly(P<0.05);those of A/G,ALT,AST,GGT,BUN,and CR in-creased extremely significantly(P<0.01);but those of ALT/AST and GLU decreased extremely significantly(P<0.01);and the other five biochemical indexes including TP,GLO,TBIL,IBIL and ALP were of no significant difference as compared with the control group(P>0.05).These results suggested that DHAV-3 was highly pathogenic to ducklings,and active prevention was required in the breeding process.

关 键 词：鸭甲型肝炎病毒3型 雏鸭 组织病理学 血清生化指标 

分 类 号：S852[农业科学—基础兽医学]