传染性支气管炎病毒反向遗传系统的构建策略与应用  

Construction strategy and application of the reverse genetic system of infectious bronchitis virus

作　　者：赵秀美 张华 贾惠 孙智远 徐孝宙[1] 余智清 姜逸[2] ZHAO Xiumei;ZHANG Hua;JIA Hui;SUN Zhiyuan;XU Xiaozhou;YU Zhiqing;JIANG Yi(Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang 212400,China;Jiangsu Institute of Poultry Sciences,Yangzhou 225125,China)

机构地区：[1]江苏农林职业技术学院,江苏镇江212400 [2]江苏省家禽科学研究所,江苏扬州225125

出　　处：《畜牧与兽医》2023年第10期109-115,共7页Animal Husbandry & Veterinary Medicine

基　　金：江苏农林职业技术学院科技项目(2021kj25);江苏农林职业技术学院大学生创新创业训练计划项目(202113103032y);江苏省自然科学基金项目(BK20201483);江苏省家禽遗传育种重点实验室开放课题资助项目(JQLAB-ZZ-202102)。

摘　　要：传染性支气管炎病毒(infectious bronchitis virus, IBV)是一种具有高度传染性的禽冠状病毒,主要感染鸡的呼吸系统、肾脏和生殖系统等,是导致家禽产业经济损失的主要原因之一。IBV血清型和基因型众多,不同毒株之间交叉保护性差,疾病防控困难。IBV反向遗传系统的建立与应用为病毒分子水平研究、致病机制研究和新型疫苗研发等提供了新的途径。本文主要介绍了IBV反向遗传技术的构建策略,包括细菌人工染色体(载体)系统、体外连接系统、痘病毒载体系统和基于靶向RNA重组技术等,以及IBV反向遗传系统在病毒基因组结构和功能研究、新型疫苗研发和病毒表达载体研究等方面的应用,以期能为IBV的研究提供参考。Infectious bronchitis virus(IBV)is a highly contagious avian coronavirus that mainly infect the respiratory,renal,and repro-ductive systems of chickens.This virus is one of the main causes of economic losses to the poultry industry.There are many serotypes and genotypes of IBV,and the cross protection among different mutants is poor,leading difficulties in the prevention and control of the disease.The establishment and application of the reverse genetic system for IBV provides new ways for the study of the virus at the molecular level,of its pathogenic mechanism,and of the development of novel vaccines against virus.This paper introduces the construction strategies of the IBV reverse genetic technology,including the bacterial artificial chromosome(vector)system,the in vitro ligation system,the vaccinia virus vec-tor system and the targeted RNA recombination technology.Also discussed in this paper are the application of the reverse genetic system for IBV in the study of the structure and function of the genome of the virus,of new vaccines development,and of the virus expression vector.The authors hope to provide reference for future research on IBV.

关 键 词：传染性支气管炎病毒 反向遗传系统 S基因 疫苗 

分 类 号：S858[农业科学—临床兽医学]