哺乳动物体外成熟卵母细胞的线粒体调控机制研究进展  

Progress in research on mitochondrial regulation mechanism of mammalian in vitro maturation oocyte

作　　者：孙立晨 许晓玲[2] 白佳桦[2] 秦玉圣 刘彦[2] 郭凯军[1] SUN Lichen;XU Xiaoling;BAI Jiahua;QIN Yusheng;LIU Yan;GUO Kaijun(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China;Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京市农林科学院畜牧兽医研究所,北京100097

出　　处：《畜牧与兽医》2023年第10期116-120,共5页Animal Husbandry & Veterinary Medicine

基　　金：十四五国家重点研发计划课题(2021YFD1200402);北京市农林科学院创新能力建设项目(KJCX20230218)。

摘　　要：卵母细胞体外成熟作为体外胚胎生产的关键技术环节,对畜牧业生产和人类辅助生殖具有重要意义。目前,该技术在生产上和临床上应用广泛,但是卵母细胞体外成熟率、受精率、优质胚胎发育率及移植后妊娠率低等问题限制了此项技术的利用效率,其中线粒体的动态分布、mtDNA拷贝数、膜电位等指标与卵母细胞发育成熟密切相关。本文简要介绍了卵母细胞成熟机制和体内外成熟差异,概括了线粒体在卵母细胞成熟过程中的调控机制以及外源线粒体调节剂在卵母细胞体外成熟体系中的应用情况和效果,以期为提高体外胚胎生产技术效率提供有力的理论依据和技术支撑。As a key technological step of in vitro embryo production,the maturation of oocytes in vitro is of great significance for animal husbandry and human assisted reproduction.At present,this technology has been widely used in production and clinical practice;but,the low rate of oocyte maturation in vitro,low fertilization rate,low high-quality embryo rate and low pregnancy rate after transplantation limit the utilization efficiency of this technology.Mitochondria dynamic distribution,mtDNA copy number,membrane potential and other indicators are closely related to oocyte development and maturation.This paper briefly introduces the mechanism of oocyte maturation,the differences in in vivo and in vitro maturation,and summarizes the regulation mechanism of mitochondria in oocyte maturation,the application and effect of ex-ogenous mitochondrial regulators in the oocyte maturation system,in order to provide an effective theoretical basis and technical support for improving the technical efficiency of in vitro embryo production.

关 键 词：线粒体 能量代谢 体外成熟 卵母细胞 哺乳动物 

分 类 号：S852[农业科学—基础兽医学]