不同pH值生物聚合物无固相钻井液中巴氏芽孢杆菌的生长预测模型  

Predictive model for the growth of Bacillus sp. in biopolymer solid⁃free drilling fluids at different pH conditions

作　　者：李之军[1,2] 杜泽华 霍力煊 武新凯 LI Zhijun;DU Zehua;HUO Lixuan;WU Xinkai(The State Key Laboratory of Geohazard Prevention and Geoenvironment Protection(Chengdu University of Technology),Chengdu Sichuan 610059,China;College of Environment and Civil Engineering,Chengdu University of Technology,Chengdu Sichuan 610059,China)

机构地区：[1]地质灾害防治与地质环境保护国家重点实验室(成都理工大学),四川成都610059 [2]成都理工大学环境与土木工程学院,四川成都610059

出　　处：《钻探工程》2023年第S01期106-112,共7页Drilling Engineering

基　　金：国家自然科学基金青年基金“微生物无固相钻井液固壁作用与机理研究”(编号:41702388)。

摘　　要：微生物诱导碳酸钙沉积(MICP)技术为地质钻探行业解决破碎地层孔壁失稳难题提供了有益借鉴,探究微生物在钻井液环境条件下的生长规律是成功应用MICP技术解决破碎地层孔壁失稳的首要前提。因此,本研究检测了pH值为7.0、7.4、7.8、8.2、8.6、9.0、9.4、9.8、10.0条件下巴氏芽孢杆菌在生物聚合物(XC)钻井液中的生长情况,对其生长规律进行分析和拟合,并构建了其生长预测模型。结果表明:在实验设定的pH值条件下,巴氏芽孢杆菌均能正常生长。综合比较各模型的回归系数、均方误差、赤池信息量准则、准确因子和偏差因子可知,修正Gompertz模型更适合描述巴氏芽孢杆菌在XC钻井液中的生长情况,Arrhenius模型更适合描述pH值对XC钻井液中巴氏芽孢杆菌的最大比生长率的影响规律。研究结果为利用MICP技术稳定破碎地层孔壁的成功应用奠定了良好的理论基础和提供了科学依据。Microbially induced calcium carbonate deposition(MICP)technology provides a useful reference for the geological drilling industry to solve the problem of borehole wall instability in fractured formations.Exploring the growth pattern of microorganisms under the environmental conditions of drilling fluids is the primary prerequisite for the successful application of MICP technology to solve borehole wall instability in fractured formations.Therefore,in this study,we examined the growth of Bacillus sp.in XC drilling fluid at pH 7.0,7.4,7.8,8.2,8.6,9.0,9.4,9.8,and 10.0,analyzed and fitted its growth pattern,and constructed its growth prediction model.The results showed that Bacillus sp.could grow normally under the experimentally set pH conditions.A comprehensive comparison of regression coefficient,mean square error,red pool information criterion,accuracy factor and bias factor of the models showed that the modified Gompertz model was more suitable to describe the growth of Bacillus sp.in XC drilling fluid,and the Arrhenius model was more suitable to describe the influence law of pH on the maximum specific growth rate of Bacillus sp.in XC drilling fluid.The results of the study lay a good theoretical foundation and provide a scientific basis for the successful application of using MICP technology to stabilize the borehole wall of fractured formations.

关 键 词：破碎地层 孔壁失稳 巴氏芽孢杆菌 MICP技术 生长预测模型 生物聚合物钻井液 

分 类 号：P634[天文地球—地质矿产勘探] TE254[天文地球—地质学]