A regulatory GhBPE-GhPRGL module maintains ray petal length in Gerbera hybrida  

作　　者：Rui Jiang Weichao Yuan Wei Yao Xuefeng Jin Xiaojing Wang Yaqin Wang 

机构地区：[1]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou 510631,China [2]Guangdong Laboratory for Lingnan Modern Agricultural,Guangzhou 510642,China

出　　处：《Molecular Horticulture》2022年第1期116-132,共17页分子园艺（英文）

基　　金：This work was supported by Laboratory of Lingnan Modern Agriculture Project(NZ2021009);Natural Science Foundation of Guangdong Province(2021A1515012479);National Key R&D Program of China(2018YFD1000404);National Natural Science Foundation of China(31672188);Guangdong Basic and Applied Basic Research Foundation(2021A1515111227).

摘　　要：The molecular mechanism regulating petal length in flowers is not well understood.Here we used transient transformation assays to confirm that GhPRGL(proline-rich and GASA-like)—a GASA(gibberellic acid[GA]stimulated in Arabidopsis)family gene—promotes the elongation of ray petals in gerbera(Gerbera hybrida).Yeast one-hybrid screening assay identified a bHLH transcription factor of the jasmonic acid(JA)signaling pathway,here named GhBPE(BIGPETAL),which binds to the GhPRGL promoter and represses its expression,resulting in a phenotype of shortened ray petal length when GhBPE is overexpressed.Further,the joint response to JA and GA of GhBPE and GhPRGL,together with their complementary expression profiles in the early stage of petal growth,suggests a novel GhBPE-GhPRGL module that controls the size of ray petals.GhPRGL promotes ray petal elongation in its early stage especially,while GhBPE inhibits ray petal elongation particularly in the late stage by inhibiting the expression of GhPRGL.JA and GA operate in concert to regulate the expression of GhBPE and GhPRGL genes,providing a regulatory mechanism by which ray petals could grow to a fixed length in gerbera species.

关 键 词：GhBPE-GhPRGL GA JA ray petal elongation Gerbera hybrida 

分 类 号：Q37[生物学—遗传学]