The SlSHN2 transcription factor contributes to cuticle formation and epidermal patterning in tomato fruit  

作　　者：Cecile Bres Johann Petit Nicolas Reynoud Lysiane Brocard Didier Marion Marc Lahaye Benedicte Bakan Christophe Rothan 

机构地区：[1]UMR 1332 BFP,INRAE,Universite de Bordeaux,33140 Villenave d’Ornon,France [2]Unite Biopolymeres,Interactions,Assemblages,INRAE,BP71627,44316 Nantes Cedex 3,France [3]Univ.Bordeaux,CNRS,INSERM,Bordeaux Imaging Center,BIC,UMS 3420,US 4,33000 Bordeaux,France [4]INRA,UMR1332 Biologie du Fruit Et Pathologie,71 Av Edouard Bourlaux,33140 Villenave d’Ornon,France

出　　处：《Molecular Horticulture》2022年第1期186-205,共20页分子园艺（英文）

基　　金：This work was supported by Bioadapt(grant ANR-13-BSV7-0012)“Adaptom”project,INRAE(TRANSFORM department)and Region Pays de la Loire(Ph.D.grant for N.R.).

摘　　要：Tomato(Solanum lycopersicum)is an established model for studying plant cuticle because of its thick cuticle covering and embedding the epidermal cells of the fruit.In this study,we screened an EMS mutant collection of the miniature tomato cultivar Micro-Tom for fruit cracking mutants and found a mutant displaying a glossy fruit phenotype.By using an established mapping-by-sequencing strategy,we identified the causal mutation in the SlSHN2 transcription factor that is specifically expressed in outer epidermis of growing fruit.The point mutation in the shn2 mutant intro-duces a K to N amino acid change in the highly conserved‘mm’domain of SHN proteins.The cuticle from shn2 fruit showed a~fivefold reduction in cutin while abundance and composition of waxes were barely affected.In addition to alterations in cuticle thickness and properties,epidermal patterning and polysaccharide composition of the cuticle were changed.RNAseq analysis further highlighted the altered expression of hundreds of genes in the fruit exocarp of shn2,including genes associated with cuticle and cell wall formation,hormone signaling and response,and transcrip-tional regulation.In conclusion,we showed that a point mutation in the transcriptional regulator SlSHN2 causes major changes in fruit cuticle formation and its coordination with epidermal patterning.

关 键 词：Mutant SHINE Cuticle EPIDERMIS Cell wall Ethylene 

分 类 号：S641.2[农业科学—蔬菜学]