Warming‑induced changes of broccoli head to cauliflower‑like curd in Brassica oleracea are regulated by DNA methylation as revealed by methylome and transcriptome co‑profiling  

作　　者：Zilei Yao Lu Yuan Ke Liu Tingjin Wang Bin Liu Yan Zhao Susheng Gan Liping Chen 

机构地区：[1]Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [2]Plant Biology Section,School of Integrative Plant Science,Cornell University,Ithaca,NY 14853,USA [3]Present address:College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China

出　　处：《Molecular Horticulture》2022年第1期318-332,共15页分子园艺（英文）

基　　金：Open access funding provided by Shanghai Jiao Tong University.This work was supported by the National Natural Science Foundation of China(Grant no.32172561 to L.P.C.);the China Postdoctoral Science Foundation(Grant no.2021 M702878 to L.Y.);the USDA NIFA(NYC-184404 to S.S.G).

摘　　要：Increasingly warming temperature impacts on all aspects of growth and development in plants.Flower development is a complex process that is very sensitive to ambient temperature,and warming temperatures often lead to abnor-mal flower development and remarkably reduce the quality and yield of inflorescent vegetables and many other crops,which can be exemplified by Brassica oleracea cv.Green Harmony F1,a broccoli cultivar,whose floral develop-ment is ceased at inflorescence meristem(at 28℃)or floral primordium stage(at 22℃),forming a cauliflower-like curd(28℃)or intermediate curd(22℃)instead of normal broccoli head at 16℃.However,the underlying molecular regulatory mechanisms are not well understood.Here we report that warming temperature(28℃ or 22℃)induced hypermethylation of the genome,especially the promoter regions of such sets of genes as ribosome biogenesis-related and others,leading to the suppression of the apex-highly-expressed distinctive genes,subsequently resulting in the abnormal floral development,as revealed by methylome and transcriptome co-profiling.The regulation of warming-induced abnormal floral development in broccoli was further verified by the fact that the DNA methylation inhibitor 5-azacytidine(5-azaC)released the expression of genes from the warming temperature-induced suppres-sion,and restored the broccoli development to normalcy at warming temperature.The research provided new approaches to breeding broccoli and other crops for growing in wider or warmer temperature zones.

关 键 词：Brassica oleracea DNA methylation Floral development cessation Global warming Heat stress 

分 类 号：S635[农业科学—蔬菜学]