线粒体靶向的次氯酸荧光探针的构建及其生物成像研究  

Mitochondrial-targeted hypochlorous acid fluorescent probe and its bioimaging

作　　者：王宏亮 张晓娟[1] 任海仙[1] WANG Hong-liang;ZHANG Xiao-juan;REN Hai-xian(Department of Chemistry,Xinzhou Normal University,Xinzhou 034000,China)

机构地区：[1]忻州师范学院化学系,山西忻州034000

出　　处：《化学研究与应用》2023年第10期2417-2423,共7页Chemical Research and Application

基　　金：国家自然科学基金项目(22277104)资助。

摘　　要：次氯酸(HClO)是生物体中重要的活性氧(ROS)物种之一,它在免疫系统中起着重要的杀菌作用,然而HClO浓度偏高与许多疾病的发生有关,如心血管疾病、关节炎、甚至癌症。本文将10-羟基苯并喹啉通过共轭双键与半花菁单元相连,构建了探针QUIN,该探针具有大的斯托克斯位移(151 nm),能以较高的灵敏度(LOD为0.13μmol·L^(-1))实现HClO的比率检测,而且探针QUIN成功检测了HeLa细胞中靶向线粒体的HClO水平,并实现了小鼠HClO水平的可视化。Hypochlorous acid(HCIO)is one of the important reactive oxygen species(ROS)species in the organism.It plays an im-portant bactericidal effect in the immune system.However,the elevated concentration of HClIO is related to many diseases,such as car-diovascular diseases,Arthritis,even cancer.In this work,10-hydroxy benzoquinoline and the hemicyanine unit were connected with conjugated double bonds to construct the probe QUIN for HCIO,which had a large Stokes shift(151 nm)and high sensitivity(LOD of 0.13μmol·L^(-1)).Probe QUIN could successfully detect the mitochondrial-targeted HCIO level of HeLa cells and visualize the HCIO level in mice.

关 键 词：次氯酸 喹啉 荧光探针 生物成像 

分 类 号：O626.23[理学—有机化学]