外源激素对仙茅未成熟种子愈伤组织诱导不定芽分化的影响  

Effects of Exogenous Hormones on Callus Induction and Adventitious Bud Differentiation of Immature Seeds of Curculigo orchioides Gaertn

作　　者：苏艳[1] 杨宝明[2] 杨超振 李永平[2] 李迅东[2] 尹可锁[2] SU Yan;YANG Bao-ming;YANG Chao-zhen(Institute of Flower,Yunnan Academy of Agricultural Sciences/Supervision and Testing Centre for Flowers of the Ministry of Agriculture and Rural Affairs/National Engineering Research Center for Ornamental Horticulture/Yunnan Yunke Flower Co.,Ltd.,Kunming,Yunnan 650100;Agricultural Environment and Resources Institute,Yunnan Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province,Kunming,Yunnan 650205;Research Institute for Tropical Eco-agricultural Sciences,Yunnan Academy of Agricultural Sciences,Yuanmou,Yunnan 651300)

机构地区：[1]云南省农业科学院花卉研究所/农业农村部花卉产品质量监督检验测试中心(昆明)/国家观赏园艺工程技术研究中心/云南云科花卉有限公司,云南昆明650100 [2]云南省农业科学院农业环境资源研究所/云南省农业跨境有害生物绿色防控重点实验室,云南昆明650205 [3]云南省农业科学院热区生态农业研究所,云南元谋651300

出　　处：《安徽农业科学》2023年第20期43-45,共3页Journal of Anhui Agricultural Sciences

基　　金：科技部、财政部国家科技资源共享服务平台项目(NCGRC-2022-47);云南省种子种业联合实验室项目(202205AR070001)。

摘　　要：为缩短仙茅育苗周期,建立高频再生体系。以未成熟种子为外植体,以MS为基本培养基,添加不同的激素浓度配比诱导产生愈伤组织,再用愈伤组织诱导出不定芽,摸索激素种类、浓度和配比对愈伤组织诱导和不定芽分化的影响,为仙茅无性繁殖体系的建立奠定基础。结果表明,仙茅未成熟种子的最佳愈伤组织诱导培养基为MS+6-BA 3.0 mg/L+NAA 0.5 mg/L,出愈率为74%;最佳不定芽分化培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,平均增殖系数达11.7。该研究可为仙茅组培快繁技术提供一种新方法和途径。In order to shorten the seedling raising period of Curculigo orchioides Gaertn.,high-frequency regeneration system was established.In this experiment,immature seeds were used as explants,with MS as the basic medium,the callus was induced by adding different hormone concentrations.Then adventitious buds were induced from callus.Through experimental research,the effects of hormone types,concentrations and ratios on callus induction and adventitious bud differentiation were explored.It laid the foundation for establishing the asexual propagation system of Curculigo orchioides Gaertn.The results showed that the best callus induction medium for immature seeds of Curculigo orchioides Gaertn.was MS+6-BA 3.0 mg/L+NAA 0.5 mg/L,the ratio on callus induction was 74%.The best adventitious bud differentiation medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,the average multiplication coefficient was 11.7.It provides a new method for tissue culture and rapid propagation of Curculigo orchioides Gaertn.

关 键 词：组织培养 快繁技术 愈伤组织 不定芽 仙茅 

分 类 号：S567.23[农业科学—中草药栽培]