猪肉中三种β-受体激动剂残留物表面增强拉曼光谱检测技术研究  

Surface-enhanced raman spectroscopy for the detection of three beta-agonist residues in pork meat

作　　者：裴佳欢 盛万里 邹明强[1] 齐小花[1] PEI Jiahuan;SHENG Wanli;ZOU Mingqiang;QI Xiaohua(Chinese Academy of Inspection and Quarantine&Key Laboratory of Food Quality and Safety for State Market Regulation,Beijing,100176,China;Faculty of Environment and Life,Beijing University of Technology;Hohhot Customs Technology Centre)

机构地区：[1]中国检验检疫科学研究院&国家市场监管重点实验室(食品质量与安全),北京100176 [2]北京工业大学环境与生命学部 [3]呼和浩特海关技术中心

出　　处：《质量安全与检验检测》2023年第5期77-82,共6页QUALITY SAFETY INSPECTION AND TESTING

基　　金：国家重点研发计划项目(2022YFF0607903);中国检验检疫科学研究院基本科研业务费项目(2022JK18);海南省重点研发计划项目(ZDYF2021SHFZ069)。

摘　　要：在畜牧生产过程中,β-受体激动剂作为一种生长促进剂,会被添加到家禽的饲料中,这些残留物含量极小,但会对人体健康造成危害。本研究基于胶体金表面等离子共振吸收性质,建立一种生鲜猪肉中β-受体激动剂的表面增强拉曼光谱检测方法。以浓度为1%的胶体金作为SERS增强基底,pH值为4的NaCl溶液作为活性剂,通过实验优化确定样品与胶体金的体积比为1∶3,使用便携式拉曼光谱仪检测猪肉中克伦特罗、莱克多巴胺、沙丁胺醇的表面增强拉曼光谱,并对不同目标物的特征峰进行振动模式归属。采用液-液萃取前处理方法,建立了猪肉中β-受体激动剂的定性定量检测方法。结果显示,3种β-受体激动剂在一定浓度范围内各自特征峰有着良好的线性关系,加标样品回收率均在80%~110%,相对标准偏差(RSD)为2.6%~8.3%,检出限低至0.001μg/g。该方法对肉类中β-受体激动剂现场检测有着广阔的应用前景,操作简便、耗时短,满足现场快速检测的需求,有着多样化的发展方向。In the process of livestock production,β-agonists,as a kind of growth promoter,are added to the feed of poultry,and these residues are present in very small amounts,but they can be hazardous to human health.In this study,a surface-enhanced Raman spectroscopy method for the determination ofβ-agonists in raw pork was developed based on the surface plasmon resonance absorption property of colloidal gold.Colloidal gold at a concentration of 1%was used as the SERS-enhanced substrate,and NaCl solution at pH 4 was used as the active agent.The volume ratio of sample to colloidal gold was determined to be 1∶3 by experimental optimisation,and a portable Raman spectrometer was used to detect the surface-enhanced Raman spectra of clenbuterol,ractopamine,and salbutamol in pork,and the vibrational modes of characteristic peaks of different targets were attributed.A qualitative and quantitative method for the determination ofβ-agonists in pork was developed using liquid-liquid extraction pretreatment,and the threeβ-agonists showed good linearity in the concentration range of their respective peaks,with the recoveries of the spiked samples in the range of 80%-110%,the relative standard deviations(RSDs)in the range of 2.6%-8.3%,and the limits of detection(LODs)in the range as low as 0.001μg/g.The method is suitable for the on-site detection ofβ-agonists in meat.This method has a broad application prospect for the on-site detection ofβ-agonists in meat,which is easy to operate,short time-consuming,and meets the needs of rapid on-site detection,and has a diversified development direction.

关 键 词：β-受体激动剂 表面增强拉曼光谱 胶体金 快速检测 

分 类 号：O657.37[理学—分析化学]