Optimization for Microbial Degumming of Ramie with Bacillus subtilis DZ_(5) in Submerged Fermentation by Orthogonal Array Design and Response Surface Methodology  

作　　者：刘芬 陈杨栋 朱鹏 曹张军 张兴群 LIU Fen;CHEN Yangdong;ZHU Peng;CAO Zhangjun;ZHANG Xingqun(College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,China;Key Laboratory of Science&Technology of Eco-Textile,Ministry of Education,Donghua University,Shanghai 201620,China;College of Textile and Clothing,Xinjiang University,Urumqi 830046,China)

机构地区：[1]College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,China [2]Key Laboratory of Science&Technology of Eco-Textile,Ministry of Education,Donghua University,Shanghai 201620,China [3]College of Textile and Clothing,Xinjiang University,Urumqi 830046,China

出　　处：《Journal of Donghua University(English Edition)》2023年第5期475-481,共7页东华大学学报（英文版）

基　　金：National Natural Science Foundation of China(No.51863020)。

摘　　要：As a kind of natural fiber,ramie fiber has distinctive advantages in textile application,but the application is limited due to the traditional degumming mode.Compared with the traditional degumming process,the microbial degumming process has many advantages.To obtain the optimal conditions for degumming ramie with Bacillus subtilis DZ_(5)(BS DZ_(5)),a combined statistical approach of orthogonal array design(OAD)and response surface methodology(RSM)was used.The influences of initial pH of the bacteria medium,culture temperature,shaking speed,degumming time and inoculum size on submerged fermentation degumming were evaluated by using fractional factorial design.The main factors in the analysis were culture temperature,shaking speed and initial pH.The residual gum mass fraction was used as the optimization index,and the optimal conditions for degumming were determined by central composite design and RSM.Thus with only a limited number of experiments,an optimal ramie microbial degumming condition was found as the culture temperature of 40℃,the initial pH in the culture medium of 8.5,the shaking speed of 205 r/min,the degumming time of 96 h and the inoculum size of 5%.After microbial degumming of ramie under the optimal conditions,there was only 10.6%residual gum by mass in the fiber.In addition,the effective degumming of BS DZ_(5)was also confirmed by a scanning electron microscope(SEM).

关 键 词：microbial degumming OPTIMIZATION RAMIE orthogonal array design(OAD) response surface methodology(RSM) 

分 类 号：TS123.2[轻工技术与工程—纺织材料与纺织品设计]