川芎指纹图谱的建立及60Co-γ辐照对其有效成分的影响  

Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60 Co-γirradiation on its active components

作　　者：邱娅璐 高鹏[1,2] 何江 陈谦[1,2] 王艳 QIU Yalu;GAO Peng;HE Jiang;CHEN Qian;WANG Yan(Sichuan Institute of Atomic Energy,Chengdu 610101,China;Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu 610101,China;College of Nuclear Technology and Automation Engineering,Chengdu University of Technology,Chengdu 610059,China)

机构地区：[1]四川省原子能研究院,成都610101 [2]辐照保藏四川省重点实验室,成都610101 [3]成都理工大学,核技术与自动化工程学院,成都610059

出　　处：《辐射研究与辐射工艺学报》2023年第5期60-72,共13页Journal of Radiation Research and Radiation Processing

基　　金：四川省科技创新苗子工程项目(2021JDRC0121,2022JDRC0120);四川省自然科学基金面上项目(2022NSFSC0593);四川省区域创新合作(2022YFQ0044);四川省科技创新人才项目(2021JDRC0042);成都市龙泉驿区科技计划项目(2022-34-2)资助~~。

摘　　要：为了建立川芎指纹图谱,考察不同剂量60Co-γ辐照对川芎8种有效成分的影响,以川芎为材料,采用SHIMADZU Shim-pack GIST C18色谱柱(250 mm×4.6 mm,5μm)对8种有效成分的标准品和对照品储备液进行分析。设定不同剂量(3 kGy、6 kGy、10 kGy、15 kGy)60Co-γ辐照处理川芎,处理后样品通过液相色谱法得到指纹图谱。采用《中药色谱指纹图谱相似度评价系统(2004A版)》对相似度进行评价。结果表明:川芎各成分相似度均大于0.988;在低于10 kGy的射线辐照处理后,川芎的8种有效成分含量无明显差异。结果表明该方法稳定可靠。该研究为辐照技术在川芎品质保障及川芎药材辐照标准的制定提供理论依据和支撑。Herein,we established the fingerprint of Ligusticum chuanxiong,and the effects of different 60Co-γirradiation doses on the eight active components of Ligusticum chuanxiong were investigated.A stock solution of the eight standard reference substances in Ligusticum chuanxiong were analyzed using a SHIMADZU Shim-pack GIST C18 chromatographic column(250 mm×4.6 mm,5μm).Different doses(3 kGy,6 kGy,10 kGy and 15 kGy)of 60Co-γirradiation were used to treat Ligusticum chuanxiong.After these treatments,the fingerprint of Ligusticum chuanxiong was obtained via liquid chromatography.The similarity was evaluated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2004 A edition),and the results indicated that the similarity of each component in Ligusticum chuanxiong was>0.988.After irradiation at<10 kGy,the contents of the eight active components in Ligusticum chuanxiong exhibited no evident differences,and the results indicated the stability and reliability of the method.Thus,this study provides a theoretical basis and support for the use of irradiation technology in the quality assurance of Ligusticum chuanxiong and the formulation of irradiation standards for Ligusticum chuanxiong.

关 键 词：川芎 60Co-γ辐照 有效成分 指纹图谱 

分 类 号：TL99[核科学技术—核技术及应用]