Gastroprotective Effect of Alpinia zerumbet(Pers.)Burttet Smith on Ethanol-induced Gastric Ulcers in vivo and vitro  

作　　者：Qing WEI Shanshan LIANG Yuechun YANG Jing ZHANG 

机构地区：[1]College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China [2]College of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China [3]Institute of Laboratory Animal Science,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China

出　　处：《Medicinal Plant》2023年第5期65-71,共7页药用植物：英文版

基　　金：Supported by Guizhou Provincial Basic Research Program(Natural Science)(ZK[2022]-492&[2020]1Y045);the National Natural Science Foundation of China(82260767).

摘　　要：[Objectives]To explore the protective effect of Alpinia zerumbet(Pers.)Burttet Smith ethanol extraction(YSJ)on gastric ulcers.[Methods]The ethanol-induced gastric ulcer model was generated by male Kunming mice.The mice were divided into six groups,including control,model(GU),positive omeprazole enteric-coated capsules(18 mg/kg),YSJ low(1.17 g/kg),medium(2.34 g/kg)and high(4.68 g/kg)dose groups.To observe the state of gastric tissues,hematoxylin and eosin(H&E)staining was applied and immunohistochemistry(IHC)was used to evaluate the expression of nuclear factor kappa-B p65(NF-κB p65),interleukin 6(IL-6),tumor necrosis factor-α(TNF-α),and Cyclooxygenase-2(COX-2)in gastric tissues.The ulcer index,biochemical parameters,and inflammatory proteins were evaluated.In vitro,GES-1 cells were induced by anhydrous ethanol to found gastric ulcer model.The groups were the same as in vivo experiment.Cytotoxicity was tested by MTT and IL-1β,TNF-α,and IL-6 were detected by Elisa assays.[Results]The injuries of gastric tissue in the model group were unambiguously observed and improved after YSJ treatment.The levels of interleukin 1 beta(IL-1β),interleukin 6(IL-6),interleukin 8(IL-8),and TNF-αin serum and tissues were decreased(P<0.05 or 0.01),whereas interleukin 4(IL-4)and prostaglandin E 2(PGE 2)were increased after treatment with YSJ(P<0.05 or 0.01).The levels of IL-1β,IL-6,TNF-α,and IL-8 were decreased in YSJ groups,while IL-4 and PGE 2 presented the opposite trend.The protein expression of p-NF-κB,NF-κB,p-IκBα,IκBα,and TNF-αwas inhibited after treatment with YSJ(P<0.05 or 0.01).[Conclusions]These results demonstrate that YSJ alleviates the occurrence and development of gastric ulcers via inhibiting inflammation.

关 键 词：Alpinia zerumbet(Pers.)Burttet Smith Gastric ulcer Inflammatory factors 

分 类 号：R285[医药卫生—中药学]