动物肠缺氧模型研究进展  

Research Progress of Intestinal Hypoxia Models in Animals

作　　者：戴文 卞苏舒 张聚民 宋厚辉 周莹珊 刘萍 王晓杜 DAI Wen;BIAN Sushu;ZHANG Jumin;SONG Houhui;ZHOU Yingshan;LIU Ping;WANG Xiaodu(China-Australia Joint Laboratory for Animal Health Big Data Analytics,Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management,Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology,Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province,College of Animal Science and Technology&College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311300,China;Development Center of Animal Husbandry and Agricultural Machinery in Hangzhou Lin′an District,Hangzhou 311300,China)

机构地区：[1]浙江农林大学动物科技学院·动物医学院、浙江省畜禽绿色生态健康养殖应用技术研究重点实验室、动物健康、互联网检测技术浙江省工程研究中心、浙江省动物医学与健康管理国际科技合作基地、中澳动物健康大数据分析联合实验室,杭州311300 [2]杭州市临安区畜牧农机发展中心,杭州311300

出　　处：《畜牧兽医学报》2023年第10期4083-4094,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：浙江省三农六方项目(2021SNLF023);浙江省农业重大技术协同推广计划项目(2021XTTGXM02);浙江农林大学科研发展基金项目(2021FR002)。

摘　　要：肠缺氧是肠组织氧气需求量高于供应量的病理现象,是多种肠道疾病的直接诱发因素、预警信号和关键特征之一。建立真实可靠的肠缺氧状态动物模型或者能模拟肠缺氧的细胞模型,对肠缺氧相关疾病的病理机制研究至关重要。本文综述了近年来适用于构建肠缺氧模型的方法,重点围绕循环性、化学性和环境性肠缺氧动物模型,物理法和化学法诱导的离体肠细胞缺氧模型和离体肠道类器官缺氧模型的构建策略、模型优劣及应用场景进行分析,为动物肠缺氧疾病的发病机制研究及治疗药物的发掘和临床药效学评价提供参考。Intestinal hypoxia is a pathological phenomenon in which the oxygen demand of intestinal tissues is higher than the oxygen supply.It is one of the direct triggers,early warning signals and key features of various intestinal diseases.The establishment of real and reliable animal models of intestinal hypoxia or cellular models that can simulate intestinal hypoxia is essential for the study of the pathological mechanisms of intestinal hypoxia-related diseases.In this paper,t The methods applicable to the construction of intestinal hypoxia models in recent years are reviewed in this paper,Focused on the construction strategies,model advantages and disadvantages,and application scenarios of models including circulatory,chemical and environmental animal models of intestinal hypoxia,physically and chemically induced cellular models of intestinal hypoxia in vitro,as well as isolated intestinal organoid models of hypoxia in vitro,in the hope of providing reference for the study of the pathogenesis of intestinal hypoxia diseases in animals and the discovery and clinical pharmacodynamic evaluation of therapeutic drugs.

关 键 词：肠缺氧 细胞模型 动物模型 肠道类器官 

分 类 号：S858[农业科学—临床兽医学]