基于基因组SNP和ROH的剑白香猪群体遗传结构解析  

Genetic Structure Analysis of Jianbai Xiang Pig Population Based on Genomic SNP and ROH

作　　者：胡紫平 王立刚[1] 宗文成 侯任达 苏艳芳 牛乃琪 王立贤[1] 王源 张龙超 HU Ziping;WANG Ligang;ZONG Wencheng;HOU Renda;SU Yanfang;NIU Naiqi;WANG Lixian;WANG Yuan;ZHNAG Longchao(Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]青岛农业大学动物科技学院,青岛266109

出　　处：《畜牧兽医学报》2023年第10期4117-4125,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2021YFD1200303-03);国家生猪产业技术体系(CARS-35);中国农业科学院科技创新工程(ASTIP-IAS02)。

摘　　要：旨在从分子水平分析剑白香猪的群体结构以及遗传多样性,对剑白香猪的种质资源鉴定和保护提供技术支持。本研究利用Porcine SNP50 BeadChip(Illumina,United States)芯片对138头剑白香猪的全基因组SNP进行扫描,通过对主成分、遗传距离、亲缘关系和公猪血统进行分析,研究剑白香猪的群体结构;通过ROH分析,确定剑白香猪的遗传多样性。结果显示,此剑白香猪群体无明显分层,但大致可以分为3个部分,群体内的遗传距离平均值约为0.273,所有公猪划分为4个血统;剑白香猪群体共检测出2893个ROHs,平均每个个体约有21个ROHs,平均ROH长度约为7.18 Mb,平均近交系数(F_(ROH))为0.27%,表明剑白香猪近交积累较少。通过多种分析表明,该群体家系数量适中,个别家系公畜数量较少,个体间亲缘关系中等,近交程度较低,群体的遗传多样性较高。本研究在基因组水平分析了剑白香猪的群体遗传结构和遗传多样性,为剑白香猪群体复壮和种业安全措施制定提供了分子依据。The purpose of this study was to analyze the population structure and genetic diversity of Jianbai Xiang pigs at the molecular level and to provide technical support for the identification and conservation of germplasm resources of Jianbai Xiang pigs.Using Porcine SNP50 BeadChip(Illumina,United States)to scan the whole genome SNP of 138 Jianbai Xiang pigs,the population structure of Jianbai Xiang pigs was studied by analyzing the principal component,genetic distance,genetic relationship and boar pedigree.The genetic diversity of the Jianbai Xiang pig was determined by ROH analysis.The results showed that there was no obvious stratification in this population,but it could be roughly divided into 3 parts.The average genetic distance within the population was about 0.273,and all boars were divided into 4 lineages.A total of 2893 ROHs were detected in Jianbai Xiang pigs,with an average of 21 ROHs per individual.The average ROH length was 7.18 Mb and the average inbreeding coefficient(F_(ROH))was 0.27%,indicating that the inbreeding accumulation of Jianbai Xiang pigs was small.A variety of analyses showed that the population had a moderate number of families,a small number of male animals in individual families,moderate genetic relationships between individuals,a low degree of inbreeding,and high genetic diversity.In this study,the population genetic structure and genetic diversity of Jianbai Xiang pigs were analyzed at the genome level,which provided the molecular basis for population rejuvenation and seed industry safety measures of Jianbai Xiang pigs.

关 键 词：剑白香猪 群体结构 ROH SNP 遗传多样性 

分 类 号：S828.2[农业科学—畜牧学]