白头翁散煎剂发酵物对感染猪流行性腹泻病毒仔猪肠道屏障功能的影响  

Effect of Pulsatilla Powder Prescription Decoction Ferments on Intestinal Barrier Function of Piglets Infected with Porcine Epidemic Diarrhea Virus

作　　者：常伟辰 李帅奇 李琰[1] 闫微 张红英[1] 王彦彬[1] 杨明凡[1] 张昂克 CHANG Weichen;LI Shuaiqi;LI Yan;YAN Wei;ZHANG Hongying;WANG Yanbin;YANG Mingfan;ZHANG Angke(College of Animal Medicine,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学动物医学院,郑州450046

出　　处：《畜牧兽医学报》2023年第10期4403-4410,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河南省重点研发项目“中药替抗关键技术研究及其在畜禽健康养殖中的应用”(221111111300)。

摘　　要：试验旨在研究白头翁散对感染猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)仔猪肠道屏障功能的影响。将24头7日龄体重相近的仔猪(杜×长×大)随机分为4组,分别为对照组、PEDV组、煎剂组和发酵组。试验期为12 d,第4天,对照组仔猪接种生理盐水,其他3组仔猪每头以10~(4.5)TCID_(50)(50%组织培养感染剂量)的剂量接种PEDV(HN2021),第5~9天,煎剂组和发酵组仔猪每天灌服煎剂和发酵液(0.2 mL·kg^(-1))。评估仔猪的生长性能、腹泻指数、病毒拷贝量、肠道细菌组成、肠道形态、肠道屏障功能,以及血清和肠道中的抗氧化指标。结果表明:煎剂和发酵液可显著提高PEDV感染仔猪平均日增重(average daily gain,ADG),降低腹泻率,抑制肠道内病毒复制(P<0.05),还可调节肠道细菌组成,显著提高乳酸杆菌和双歧杆菌数量(P<0.05);给药缓解了PEDV感染造成的仔猪肠道绒毛高度(villus height,VH)显著下降和隐窝深度(crypt depth,CD)增加,改善肠道形态,显著提高血清中D-木糖含量,降低肠型脂肪酸结合蛋白(I-FABP)浓度,从而增强肠道屏障功能(P<0.05)。此外,煎剂和发酵液可降低氧化应激,表现为总超氧化物歧化酶(T-SOD)活性显著升高,丙二醛(MDA)含量显著降低(P<0.05)。这些数据表明白头翁散可增强感染PEDV仔猪的肠道屏障功能。The trial aimed to study the effect of Pulsatilla Powder prescription on the intestinal barrier function of piglets infected with porcine epidemic diarrhea virus(PEDV).Twenty-four 7-day-old piglets(du×long×large)were randomly divided into 4 groups:control,PEDV,decoction and fermentation groups.The test period was 12 days.On day 4,piglets in the control group were inoculated with saline and the other three groups were inoculated with PEDV(HN2021)at a dose of 104.5 TCID 50(50%tissue culture infection dose)per pig,and on days 5-9,the decoction and fermentation groups were orally given decoction and fermentation solution(0.2 mL·kg^(-1))daily.To evaluate the growth performance,diarrhea index,viral copies,intestinal bacterial composition,intestinal morphology,intestinal barrier function of piglets,and antioxidant indicators in the serum and intestine.The results showed that the decoction and fermentation solution significantly increased the average daily gain(ADG),reduced diarrhea rate and inhibited viral replication in the intestine of the PEDV-infected piglets(P<0.05);and also regulated the intestinal bacteria composition of piglets,significantly increased the number of Lactobacillus and Bifidobacterium(P<0.05);the administration alleviated the significant decrease in intestinal villus height(VH)and increase in crypt depth(CD)in piglets caused by PEDV infection,improved intestinal morphology,significantly increased D-xylose content and decreased fatty acid binding protein(I-FABP)concentration in serum,thereby enhancing intestinal barrier function(P<0.05).In addition,the decoction and fermentation solution reduced oxidative stress,manifested by significantly increased total superoxide dismutase(T-SOD)activity and decreased malondialdehyde(MDA)content(P<0.05).These dates indicate Pulsatilla Powder can enhance intestinal barrier function in PEDV-infected piglets.

关 键 词：白头翁散 发酵 PEDV 生长性能 肠道屏障功能 抗氧化功能 

分 类 号：S853.9[农业科学—临床兽医学]