刈割强度对晋北盐渍化草地土壤细菌群落结构的影响  

Effects of Mowing Intensity on Soil Bacterial Community Structure in Salinized Grassland of Northern Shanxi Province

作　　者：欧俊 郝杰[3] 王犇 任歆玥 米佳 董宽虎[3,5] OU Jun;HAO Jie;WANG Ben;REN Xinyue;MI Jia;DONG Kuanhu(Institute of Loess Plateau,Shanxi Key Laboratory for Ecological Restoration of Loess Plateau China,Shanxi University,Taiyuan 030006,China;Xinfeng Middle School of Jiangxi Province,Xinfeng 341699,China;College of Grassland Science,Shanxi Agricultural University,Taigu 030801,China;Field Scientific Observation and Research Station of the Ministry of Education of Shanxi Subalpine Grassland Ecosystem,Ningwu 036700,China;Observation and Research Station for Grassland Ecosystem in the Loess Plateau,Youyu 037200,China)

机构地区：[1]山西大学黄土高原研究所,黄土高原生态恢复山西省重点实验室,山西西太原030006 [2]江西省信丰中学,江西信丰341699 [3]山西农业大学草业学院,山西太谷030801 [4]山西亚高山草地生态系统教育部野外科学观测研究站,山西宁武036700 [5]山西右玉黄土高原草地生态系统定位观测研究站,山西右玉037200

出　　处：《山西大学学报（自然科学版）》2023年第5期1205-1216,共12页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省基础研究计划资助项目(202203021211303);国家自然科学基金(31572452)。

摘　　要：刈割是农牧交错带主要的草地利用方式,为探究刈割对草地土壤细菌群落的影响,本研究通过土壤微生物高通量测序研究不同刈割强度下土壤细菌多样性和群落结构。结果表明,刈割处理增加了细菌群落的丰富度、多样性和优势度。在中度刈割强度下,土壤细菌的Shannon-Wiener多样性指数显著高于其他处理,达到6.505;刈割能使Simpson指数增加13.89%~19.44%,在轻度刈割强度下土壤细菌的Simpson指数最高达到0.0043。盐渍化草地的土壤细菌优势类群有变形菌门(Proteobacteria)、放线菌门(Actinobacteriota)、酸杆菌门(Acidobacteriota)、厚壁菌门(Firmicutes)。刈割显著降低了变形菌门的相对丰度,并且在中度刈割强度下相对丰度值最低为21.09%;显著增加了放线菌门和厚壁菌门的相对丰度,均在中度刈割强度下丰度最高,分别为25.95%和11.35%。土壤因子(硝态氮、全碳、碳氮比和水分)影响土壤细菌群落组成,刈割可能通过影响土壤全碳驱动土壤细菌中优势类群的改变,进而影响土壤细菌多样性。Mowing is an important way to use grassland of agro-pastural ecotone in northern China.In order to investigate the ef-fects of mowing on soil bacterial community in salinized grassland,the soil bacterial diversity and community structure under differ-ent mowing intensities were studied by high-flux sequencing of soil microorganisms.The results showed that mowing treatments in-creased the abundance,diversity and dominance of bacterial community.At moderate mowing intensity,the Shannon-Wiener diver-sity index of soil bacteria was significantly higher than that of other treatments,reaching 6.505.Mowing treatments could increase the Simpson index by 13.89%to 19.44%.And the Simpson index of soil bacteria community was up to 0.0043 under light mowing treatment conditions.The dominant groups of soil bacteria in salinized grassland were Proteobacteria,Actinobacteriota,Acidobacteriota and Firmicutes.The relative abundance of Proteobacteria was significantly reduced by mowing treatments,and the value was at least 21.09% at moderate mowing intensity.The relative abundance of Actinobacteriota and Firmicutes were significantly increased by mowing treatments.Under moderate mowing treatment,both of which had the highest relative abundance at 25.95% and 11.35%,respectively.The soil factors,including nitrogen,total carbon,carbon-to-nitrogen ratio and soil moisture,affected the composition of soil bacterial community.The mowing might interfere soil bacterial diversity by affecting the changes in the dominant groups of soil bacteria driven by the soil total carbon.

关 键 词：刈割强度 农牧交错带 草地生态系统 土壤细菌群落 

分 类 号：S812.2[农业科学—草业科学] Q938.1[农业科学—畜牧学]