CaCl_(2)、甜菜碱、5-氨基乙酰丙酸提升番茄耐低温弱光研究  

Study of the Chilling and Low Light Tolerance of Tomato Improved by CaCl_(2),Glycine Betaine,and 5-Aminolevulinic Acid

作　　者：苏常红 寻雅雯 宋子豪 SU Changhong;XUN Yawen;SONG Zihao(College of Geographic and Environmental Sciences,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学地理与环境科学学院,天津300387

出　　处：《山西大学学报（自然科学版）》2023年第5期1217-1226,共10页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(42271302)。

摘　　要：前期研究中筛选出对番茄幼苗耐低温弱光交互胁迫有促进作用的三种化学物质:1 mmol/L的CaCl_(2)、2.5mmol/L甜菜碱(GB)、30 mg/L的5-氨基乙酰丙酸(ALA),本研究在此基础上对三种化学物质在增强番茄苗耐低温与弱光胁迫的贡献及机理进行了进一步研究。结果表明,喷施三种化学物质能显著提升番茄幼苗耐低温能力,幼苗净光合速率和气孔导度显著高于对照,冷害指数低于对照,尤以CaCl_(2)处理的效果最好。三种物质可以显著提高番茄耐弱光能力,净光合速率、气孔导度、叶绿素相对含量(SPAD)高于空白对照组(CK),尤以ALA增强耐弱光效果最好。对三种化学物质耐低温弱光机理进行了研究,结果表明CaCl_(2)、GB、ALA能够增强番茄幼苗低温弱光下过氧化物歧化酶(SOD)保持较高活性,丙二醛(MDA)含量降低,细胞膜渗漏率降低。三种化学物质还能够使番茄植株渗透调节物质脯氨酸含量保持较高水平。在叶绿素含量方面,三种化学物质有助于番茄幼苗在低温弱光下保持较高的叶绿素总量水平,尤其是保持较高的叶绿素b含量。In previous study,it is found that CaCl_(2),of 1 mmol/L,glycine betaine of 2.5 mmol/L,and 5-aminolevulinic acid(ALA)of 30 mg/L were effective in enhancing the resistance of tomato seedlings to chilling and low light.In this study,the contributions and underlying mechanisms of these three compounds to resist either chilling or low light for tomato seedlings were further studied.The enhanced resistance to chilling was reflected by higher net photosynthesis rate(Pn),higher stomata conductivity(Cs),and lower withering index than that of CK,particularly for CaCl_(2).The enhanced resistance to low light was indicated by higher Pn,higher Cs,and higher relative chlorophyll content(SPAD)values than that of control check(CK),particularly for ALA.Mechanism research shows that these three compounds can enhance superoxide dismutase(SOD)action,decrease Malondialdehyde(MDA)content,and alleviate cell membrane electrolyte leakage.Furthermore,these compounds help retain high level of proline,a major osmotic regula-tor in cells.Finally,the three compounds can retain high level of chlorophyll,particularly the chlorophyll B.

关 键 词：番茄 CaCl_(2) 甜菜碱 5-氨基乙酰丙酸 低温 弱光 

分 类 号：S641.2[农业科学—蔬菜学]