健康大学生多环芳烃内暴露与肺功能变化的关系  

Relationship between polycyclic aromatic hydrocarbons internal exposure and lung function change among healthy collge students

作　　者：陈俊杰 刘思巧 李淼 郝珂璐 张艳丽 李卫红 徐厚君 郝玉兰 王曼曼 王学生 王茜 CHEN Junjie;LIU Siqiao;LI Miao;HAO Kelu;ZHANG Yanli;LI Weihong;XU Houjun;HAO Yulan;WANG Man-man;WANG Xuesheng;WANG Qian(School of Public Health,North China University of Science and Technology,Tangshan(063000),Hebei Province,China)

机构地区：[1]华北理工大学公共卫生学院,河北唐山063000 [2]华北理工大学心理与精神卫生学院 [3]华北理工大学实验动物中心

出　　处：《中国学校卫生》2023年第9期1409-1413,1417,共6页Chinese Journal of School Health

基　　金：河北省自然科学基金面上项目(H2021209054,H2022209025);唐山市科技计划项目(19130209g)。

摘　　要：目的研究尿中单羟基多环芳烃代谢物(OH-PAHs)与肺功能之间的关系以及氧化应激在其中的中介作用,为空气污染治理与政策制定提供科学依据。方法招募华北理工大学45名健康大学生为研究对象,分别于2017年11月至2018年10月进行4次随访调查和健康体检,测量肺功能参数[用力肺活量(FVC)、1 s用力呼气量(FEV1)、呼气峰值流速(PEF)、FEV1/FVC和用力呼气流量(FEF25%~75%)]和尿液中7种暴露标志物OH-PAHs(∑7OH-PAHs)、氧化应激标志物8-羟基-2’-脱氧鸟苷(8-OHdG)、8-异前列腺素F2α(8-iso-PGF2α)。利用线性混合效应模型分析尿多环芳烃代谢物与肺功能之间的关系,并用中介模型评估氧化应激在OH-PAHs与肺功能之间关系中的中介效应。结果研究对象FVC、FEV1、FEV1/FVC、PEF和FEF25%~75%的中位数分别为4.37 L,3.58 L,83.00%,4.38 L/s和3.32 L/s。2-羟基芴(2-OHFlu)的对数转换值每增加1个(ln)单位,FVC下降5.05%(β%=-5.05%,95%CI=-8.85%~-1.09%),FEV1下降4.15%(β%=-4.15%,95%CI=-7.94%~-0.22%),FEF25%~75%下降5.87%(β%=-5.87%,95%CI=-11.35%~-0.05%)。2-OHFlu和9-羟基菲(9-OHPhe)的对数转换值每增加1个(ln)单位,PEF分别降低7.03%(β%=-7.03%,95%CI=-12.60%~-1.11%)和7.08%(β%=-7.08%,95%CI=-13.50%~-0.17%)。此外,尿∑7OH-PAHs与尿8-OHdG和8-iso-PGF2α水平呈正相关(r值分别为0.64,0.69,P值均<0.01)。同时,8-OHdG介导2-OHFlu与FVC和FEV1的相关性分别为17.06%和15.71%。结论健康大学生尿中OH-PAHs与肺功能呈负相关,8-OHdG在2-OHFlu与FVC和FEV1之间的关系中起介导作用。应积极制定相关政策治理空气污染,维护青年人的健康生活状态。Objective To investigate the relationship between urinary monohydroxylated metabolites of hydroxyl polycyclic aro-matic hydrocarbons(OH-PAHs)and lung function,as well as the role of oxidative stress in these associations,so as to provide a scientific basis for air pollution control and policy formulation.Methods A panel study was carried out among 45 young healthy a-dults.Four fllow-up surveys and health examinations were conducted from November 2017 to October 2018 to measure lung func-tion parameters[forced vital capacity(FVC),second forced expiratory volume in one second(FEV1),peak expiratory flow(PEF),FEV1/FVC,and forced expiratory flow between 25%and 75%vital capacity(FEF_(25%-75%)),markers of exposure to 70H-PAHs[∑_(7)OH-PAHs],and markers of oxidative stress[8-hydroxy-2'-deoxyguanosine(8-OHdG)and 8-isoprostaglandin-F_(2α)(8-iso-PGF_(2α))].The relationship between urinary PAH metabolites and lung function was quantified by linear mixed effects models.Medi-ation analysis was performed to assess the role of oxidative stress in the relationship between OH-PAHs and lung function.Results The median values of FVC,FEV1,FEVV/FVC,PEF,and FEF_(25%-75%)were 4.37 L,3.58 L,83.00%,4.38 L/s,and 3.32 L/s,respectively.The results showed that each 1-unit increase in log-transformed value of 2-Hydroxyfluorene(2-OHFlu)was associated with a 5.05%decrease(β%=-5.05%,95%CI=-8.85%--1.09%)in FVC,4.15%decrease(β%=-4.15%,95%CI=-7.94%-0.22%)in FEV1 and 5.87%decrease(B%=-5.87%,95%CI=-11.35%-0.05%)in FEF_(25%-75%),respectively.Each 1-unit in-crease in log-transformed values of 2-0HFlu and 9-Phenanthrol(9-0HPhe)was associated with a 7.03%decrease(β%=-7.03%,95%CI=-12.60%--1.11%)and a 7.08%decrease(β%=-7.08%,95%CI=-13.50%--0.17%)in PEF,respectively.Addition-ally,urinary∑_(7)OH-PAHs had a positive correlation with the levels of urinary 8-OHdG and 8-iso-PCF2α(r=0.64,0.69,P<0.01).Meanwhile,the levels of 8-0HdG mediated 17.06%and 15.71%of the association between 2-0HFlu with FVC and FEV1.Conclusion The finding reveales a negative rela

关 键 词：多环芳烃 肺 氧化性应激 学生 线性模型 

分 类 号：R816.41[医药卫生—放射医学] R179[医药卫生—临床医学] R193