红景天苷对高原红细胞增多症模型大鼠骨髓CD71+有核红细胞凋亡的影响  

Effect of salidroside on apoptosis of CD71+nucleated red blood cells in bone marrow in high altitude polycythemia model rats

作　　者：郭勇 王生艳 易静静 崔森[3] GUO Yong;WANG Shengyan;YI Jingjing;CUI Sen(Graduate School,Qinghai University,Xining 810000,China;Department of Clinical Medical Sciences,Qinghai Health Vocational and Technical College,Xining 810000,China;Department of Hematology,Affiliated Hospital,Qinghai University,Xining 810000,China)

机构地区：[1]青海大学研究生院,青海西宁810000 [2]青海卫生职业技术学院临床医学系,青海西宁810000 [3]青海大学附属医院血液科,青海西宁810000

出　　处：《吉林大学学报（医学版）》2023年第5期1174-1181,共8页Journal of Jilin University：Medicine Edition

基　　金：青海省科技厅自然科学基金项目(2019-ZJ-902)。

摘　　要：目的:探讨红景天苷对高原红细胞增多症(HAPC)模型大鼠骨髓CD71+有核红细胞(RBC)凋亡的影响,并阐明其作用机制。方法:45只SD雄性大鼠随机分为对照组(海拔1500 m条件+NaCl溶液)、模型组(海拔5000m条件+NaCl溶液)和药物组(海拔5000 m条件+0.15 g·kg^(-1)·d^(-1)红景天苷),每组15只。检测各组大鼠血常规中RBC数、血红蛋白(Hb)水平和红细胞比容(HCT)水平,流式细胞术检测各组大鼠骨髓CD71+有核RBC百分率、骨髓CD71+有核RBC凋亡率、骨髓CD71+有核RBC中线粒体膜电位(MMP)和含半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)蛋白表达水平,Western blotting法检测各组大鼠骨髓CD71+有核RBC中B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)和含半胱氨酸的天冬氨酸蛋白水解酶9(Caspase-9)蛋白表达水平。结果:与对照组比较,模型组大鼠外周血中RBC数、Hb水平和HCT水平均升高(P<0.01),骨髓CD71+有核RBC百分率和骨髓CD71+有核RBC凋亡率升高(P<0.01),骨髓CD71+有核RBC中Bax、Caspase-9和Caspase-3蛋白表达水平升高(P<0.05)。与模型组比较,药物组大鼠外周血中骨髓CD71+有核RBC百分率均明显降低(P<0.01),骨髓CD71+有核RBC百分率和骨髓CD71+有核RBC凋亡率降低(P<0.01),骨髓CD71+有核RBC中MMP水平升高(P<0.05),Caspase-3、Bax和Caspase-9蛋白表达水平降低(P<0.01)。结论:红景天苷可有效抑制HAPC大鼠骨髓有核RBC凋亡增加,并改善低氧引起的RBC过度积累,可能在大鼠HAPC发病过程起预防和保护作用。Objective:To discuss the effect of salidroside on the apoptosis of the bone marrow CD71+nucleated red blood cells(RBC)in the rat model of high altitude polycythemia(HAPC),and to clarify its mechanism.Methods:Forty-five male SD rats were randomly divided into control group(exposed to 1500 m altitude+NaCl solution),model group(exposed to 5000 m altitude+NaCl solution),and drug group(exposed to 5000 m altitude+0.15 g·kg^(-1)·d^(-1) salidroside),and there were 15 rats in each group.The RBC counts,hemoglobin(Hb)levels,and hematocrit(HCT)levels of the rats in various groups were detected;flow cytometry was used to detect the percentages of bone marrow CD71+nucleated RBC,apoptotic rates of bone marrow CD71+nucleated RBC,levels of mitochondrial membrane potential(MMP)and expression levels of cysteinyl aspartate-specific protease-3(Caspase-3)in bone marrow CD71+nucleated RBC of the rats in various groups;Western blotting method was used to detect the expression levels of B-cell lymphoma-2(Bcl-2),Bcl2-associated X protein(Bax),and cysteinyl aspartatespecific protease-9(Caspase-9)proteins in bone marrow CD71+nucleated RBC of the rats in various groups.Results:Compared with control group,the RBC count,Hb level,HCT level of the rats in model group were increased(P<0.01),and the percentage and apoptotic rate of bone marrow CD71+nucleated RBC were increased(P<0.01),and the expression levels of Bax,Caspase-9,and Caspase-3 proteins in the bone marrow CD71+nucleated RBC were increased(P<0.05).Compared with model group,the percentage of bone marrow CD71+nucleated RBC of the rats in drug group was decreased(P<0.01),the apoptotic rate of bone marrow CD71+nucleated RBC(P<0.01),and the MMP level in the bone marrow CD71+nucleated RBC were increased(P<0.05),and the expression levels of Caspase-3,Bax,and Caspase-9 proteins in the bone marrow CD71+nucleated RBC were decreased(P<0.01).Conclusion:Salidroside can effectively inhibit the increasing of apoptosis of nucleated RBC in bone marrow of the HAPC rats and improve the excessive accumu

关 键 词：高原红细胞增多症 红景天苷 骨髓单个核细胞 细胞凋亡 红细胞 

分 类 号：R551.3[医药卫生—血液循环系统疾病]