2型糖尿病患者外周血淋巴细胞中CDKAL1基因及其剪接异构体的表达水平和临床意义  

Expressions of CDKAL1 gene and its splice isomers in peripheral blood lymphocytes of patients with type 2 diabetes mellitus and their clinical significances

作　　者：王嘉欣[1] 王珍琦 张萱[3] WANG Jiaxin;WANG Zhenqi;ZHANG Xuan(Department of Endocrinology,Second Hospital,Jilin University,Changchun 130041,China;Key Laboratory of Radiobiology,National Health Commission,School of Public Health,Jilin University,Changchun 130021,China;Department of Science and Technology,Second Hospital,Jilin University,Changchun 130041,China)

机构地区：[1]吉林大学第二医院内分泌科,吉林长春130041 [2]吉林大学公共卫生学院国家卫健委放射生物学重点实验室,吉林长春130021 [3]吉林大学第二医院科研部,吉林长春130041

出　　处：《吉林大学学报（医学版）》2023年第5期1290-1295,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省财政厅卫生专项项目(201817275775);吉林省卫健委卫生健康科技能力提升项目(2021JC039)。

摘　　要：目的:探讨2型糖尿病(T2DM)患者外周血淋巴细胞中细胞周期蛋白依赖性激酶5调节亚基相关蛋白1类似物1(CDKAL1)基因及其剪接异构体的表达水平,并探讨其临床意义。方法:收集65例糖尿病患者,其中T2DM患者20例(T2DM组)、糖尿病肾病(DN)患者23例(DN组)和糖尿病视网膜病变(DR)患者22例(DR组),并选择同期健康体检者28名(健康对照组)。采用实时荧光定量PCR(RT-qPCR)法检测各组研究对象外周血淋巴细胞中CDKAL1基因及其2种剪接异构体CDKAL1-X1和CDKAL1-X2表达水平,分析其表达与T2DM及其糖尿病微血管并发症的关系。结果:与健康对照组比较,T2DM组患者外周血淋巴细胞中CDKAL1基因表达水平升高(Z=4.705,P<0.01),CDKAL1-X1表达水平升高(Z=2.698,P=0.007)。与健康对照组比较,DR组和DN组患者外周血淋巴细胞中CDKAL1基因和CDKAL1-X1表达水平升高(P<0.05);DR组患者外周血淋巴细胞中CDKAL1基因表达水平高于DN组(P<0.05)。结论:T2DM患者外周血淋巴细胞中CDKAL1基因和CDKAL1-X1表达水平升高可能参与糖尿病及其并发症的发生发展。Objective:To discuss the expression levels of the cyclin-dependent kinase 5 regulatory subunit associated protein 1-like 1(CDKAL1)gene and its splice isoforms in peripheral blood lymphocytes of the patients with type 2 diabetes mellitus(T2DM),and to clarify their clinical significances.Methods:A total of 65 diabetes mellitus patients were enrolled,including 20 T2DM patients(T2DM group),23 diabetic nephropathy(DN)patients(DN group),22 diabetic retinopathy(DR)patients(DR group),and 28 healthy examiners at the same term(healthy control group).Real-time fluorescence quantitative PCR(RT-qPCR)method was used to detect the expression levels of CDKAL1 gene and its two splice isoforms,CDKAL1-X1 and CDKAL1-X2,in peripheral blood lymphocytes of the subjects in various groups.The relationships between the clinical significances of their expressions and T2DM and its microvascular complications were analyzed.Results:Compared with healthy control group,the expression levels of CDKAL1 gene(Z=4.705,P<0.01)and CDKAL1-X1(Z=2.698,P=0.007)in peripheral blood lymphocytes of the patients in T2DM group were increased.Compared with healthy control group,the expression levels of CDKAL1 gene and CDKAL1-X1 in peripheral blood lymphocytes of the patients in DR and DN groups were increased(P<0.05);the expression level of CDKAL1 gene in peripheral blood lymphocytes of the patients in DR group was higher than that in DN group(P<0.05).Conclusion:The high expressions of CDKAL1 gene and CDKAL1-X1 in peripheral blood lymphocytes of the T2DM patients may play an important role in the occurence and development of diabetic mellitus and its complications.

关 键 词：糖尿病 2型 细胞周期蛋白依赖性激酶5调节亚基相关蛋白1类似物1 基因表达 糖尿病肾病 糖尿病视网膜病变 

分 类 号：R587.1[医药卫生—内分泌]