HIV-1广谱中和抗体筛选技术的研究进展  

Research Progress of the Screening Technique for HIV-1 Broad-spectrum Neutralizing Antibodies

作　　者：蒋雯玲 邓婷婷 李少伟[1,2] 顾颖[1,2] JIANG Wen-ling;DENG Ting-ting;LI Shao-wei;GU Ying(National Institute of Diagnostics and Vaccine Development in Infectious Diseases,School of Public Health,Xiamen University,Xiamen 361102,China;State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,School of Life Sciences,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门大学公共卫生学院,国家传染病诊断试剂与疫苗工程技术研究中心,厦门361102 [2]厦门大学生命科学学院,分子疫苗学与分子诊断学国家重点实验室,厦门361102

出　　处：《中国生物工程杂志》2023年第9期46-54,共9页China Biotechnology

基　　金：国家自然科学基金(82171821)。

摘　　要：HIV-1广谱中和抗体(HIV-1 bNAbs)是一类可以中和大多数流行株的抗体。HIV-1 bNAbs的研究可以为抗艾滋病药物提供候选分子和为艾滋病疫苗设计提供指导,是评估艾滋病疫苗效果的重要指标之一。HIV-1 bNAbs可通过传统筛选技术获得,如杂交瘤技术、EBV转化和展示库技术等。近年来,随着单细胞克隆和分选技术的发展,HIV-1 bNAbs的筛选效率和抗体特异性显著提高。多项技术结合的筛选手段和新型筛选技术LIBRA-seq,以及生物信息学辅助的筛选技术将抗体序列和功能信息统一起来,为HIV-1 bNAbs筛选和疫苗设计提供技术支持。除了HIV-1,这些筛选技术和方法也可用于其他病毒bNAbs的筛选,为疫苗设计和抗病毒药物开发提供了有益启示。综述了广泛应用于HIV-1 bNAbs的筛选技术和最新进展,为后续HIV-1或其他病毒bNAbs的筛选提供参考。HIV-1 broadly neutralizing antibodies(HIV-1 bNAbs)are a class of antibodies that can neutralize most of the circulating strains.The study of HIV-1 bNAbs can provide candidates for anti-AIDS drugs and guide vaccine design,and meanwhile HIV-1 bNAbs is an important indicator for evaluating the efficacy of HIV-1 vaccines.HIV-1 bNAbs can be obtained through traditional screening techniques,such as hybridoma technology,Epstein-Barr virus transformation,and the display library technology.In recent years,with the development of single-cell cloning and sorting technologies,the screening efficiency and antibody specificity of HIV-1 bNAbs have significantly improved.Combined screening methods and novel screening technologies,such as LIBRA-seq and bioinformatics-assisted screening techniques,can unify antibody sequences and functional information,providing technical support for HIV-1 bNAb screening and vaccine design.In addition,these screening techniques and methods for HIV-1 can also be used for the screening of bNAbs against other viruses,providing useful insights into vaccine design and antiviral drug development.This article reviews the widely used screening techniques and latest advances in HIV-1 bNAbs,providing a reference for the screening of HIV-1 or other viruses’bNAbs in the future.

关 键 词：HIV-1广谱中和抗体 筛选技术 LIBRA-seq 生物信息学 

分 类 号：Q819[生物学—生物工程]