参附注射液联合血必净对绵羊多脏器功能障碍综合征PCT和HLA-DR水平的影响  

Effect of Shenfu injection combined with Xuebijing on the levels of PCT and HLA-DR in sheep with multiple organ dysfunction syndrome

作　　者：王红义 冯小明[2] 李渊明 肖攀 陆璐[1] 申栋帅 牛廷献 张萍[4] WANG Hongyi;FENG Xiaoming;LI Yuanming;XIAO Pan;LU Lu;SHEN Dongshuai;NIU Tingxian;ZHANG Ping(Basic Medical Laboratory,No.940 Hospital of Joint Logistics Support Force of PLA,Lanzhou 730050,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;Department of Neurology,Gansu Central Hospital,Lanzhou 730070,China;Department of Clinical Laboratory,No.940 Hospital of Joint Logistics Support Force of PLA,Lanzhou 730050,China)

机构地区：[1]中国人民解放军联勤保障部队第940医院基础医学实验室,兰州730050 [2]中国农业科学院兰州兽医研究所,兰州730046 [3]甘肃省中心医院神经内科,兰州730070 [4]中国人民解放军联勤保障部队第940医院检验科,兰州730050

出　　处：《实验动物科学》2023年第5期7-11,共5页Laboratory Animal Science

基　　金：军队实验动物专项科研课题(SYDW[2020]27,SYDW[2018]12);甘肃省自然科学基金实验动物专项(22JR5RA023,23JRRA536)。

摘　　要：目的探讨参附注射液联合血必净(XBJ)对高海拔地区内毒素致绵羊多脏器功能障碍综合征(MODS)、血浆清降钙素原(PCT)和MHC-II类分子HLA-DR水平的影响。方法将24只绵羊随机分为正常组、模型组、参附注射液组(观察1组)及参附注射液联合血必净组(观察2组),每组6只。除正常组外,其余各组采用静脉泵入脂多糖LPS(6μg/kg,30 min)建立MODS动物模型,观察1组给予参附注射液静脉泵入(100 mL,20 mL/h),观察2组静脉泵入参附注射液(100 mL,20 mL/h)和血必净(Xuebijing,XBJ)(2 mL/kg)。观察1组和观察2组皆以1周为1疗程,共计1疗程。建模后及干预后0.5、1、2、3、7 d,静脉采血检测PCT、HLA-DR。结果与正常组比较,模型组血浆PCT水平降低,而HLA-DR升高,差异有统计学意义(P<0.05);参附注射液、参附注射液+XBJ组绵羊的血浆PCT水平明显降低(P<0.05),且观察2组下降最为显著(P<0.05);观察1组、观察2组血浆HLA-DR较模型组均有所升高(P<0.05),且观察2组升高最为明显(P<0.05)。结论参附注射液联合血必净(XBJ)对高海拔地区内毒素致绵羊多脏器功能障碍综合征(MODS)动物体征的改善,对肺、肝和肾等多脏器损伤的防治作用可能与降低血浆PCT,升高血浆HLA-DR水平有关。Objective To investigate the effects of Shenfu injection combined with Xuebijing(XBJ)on endotoxin-induced multiple organ dysfunction syndrome(MODS),plasma procalcitonin(PCT)and MHC-II molecule HLA-DR in high altitude areas Level of influence.Method 24 sheep were randomly divided into normal group,model group,Shenfu injection group(observation 1 group)and Shenfu injection combined with Xuebijing group(observation 2).Three sheep were taken as the blank control group.Group of 6 only.Except for the normal group,the other groups used intravenous pumping of lipopolysaccharide LPS(6μg/kg,30 min)to establish a MODS animal model.Observation group 1 was given intravenous pumping of Shenfu injection(100 mL,20 mL/h),Shenfu injection(100 mL,20 mL/h)and Xuebijing(XBJ)(2 mL/kg)were injected intravenously in observation group 2.Observation group 1 and observation group 2 took 1 week as a course of treatment,a total of 1 course of treatment.After model building and 0.5,1,2,3,7 d after intervention,venous blood was collected to detect PCT and HLA-DR.Result Compared with the normal group,the plasma PCT level of the model group decreased,while the HLA-DR increased,the difference was statistically significant(P<0.05);the plasma PCT level of sheep in the Shenfu injection and Shenfu injection+XBJ group was significantly reduced(P<0.05),and the decrease in the observation group 2 was the most significant(P<0.05);the plasma HLA-DR of the observation group 1 and the observation group 2 were both higher than the model group(P<0.05),And the observation of the two groups was the most obvious(P<0.05).Conclusion Shenfu injection combined with Xuebijing(XBJ)can improve the animal signs of multiple organ dysfunction syndrome(MODS)caused by endotoxin in high altitude area,and prevent and treat multiple organ damages such as lung,liver and kidney.It may be related to the reduction of plasma PCT and the increase of plasma HLA-DR levels.

关 键 词：内毒素 多脏器功能障碍综合征 参附注射液 血必净 血清降钙素原 

分 类 号：Q95-3[生物学—动物学]