仙灵骨葆胶囊对生长期大鼠骨强度的影响  

Effect of Xianling Gubao Capsule on Bone Strength of Growing Rats

作　　者：高玉海[1,2] 谢高倩 孙薇 吴西 王中琪 柏鑫 魏朋 王龙飞 陈克明[1,2] GAO Yuhai;XIE Gaoqian;SUN Wei;WU Xi;WANG Zhongqi;BAI Xin;WEI Peng;WANG Longfei;CHEN Keming(Fundamental Medical Science Research Laboratories of the 940 Hospital of Chinese PLA Joint Logistics Support Force,Lanzhou 730050,China;Gansu Key Laboratory of Stem Cells and Genetic Drugs,Lanzhou 730050,China;Department of Pharmacy,the General Hospital of Chinese PLA Western Theater Area Command,Chengdu 610083,China)

机构地区：[1]中国人民解放军联勤保障部队第940医院基础医学实验室,兰州730050 [2]甘肃省干细胞与基因药物重点实验室,兰州730050 [3]中国人民解放军西部战区总医院药剂科,成都610083

出　　处：《实验动物科学》2023年第5期19-23,共5页Laboratory Animal Science

基　　金：联勤保障部队第九四○医院骨稳态调控与转化应用重点实验室(2021yxky081);联勤保障部队第九四○医院青年培育项目(2021yxky053);甘肃省自然科学基金(22JR11RA013)。

摘　　要：目的研究仙灵骨葆胶囊对生长期大鼠骨强度的影响。方法将20只1月龄SPF级SD雌性大鼠按随机数字表法分为对照组和仙灵骨葆组,每组10只。仙灵骨葆组每天灌服378 mg/kg仙灵骨葆胶囊,对照组每天给予等体积蒸馏水。实验期间每两周测一次全身骨密度,待第6周仙灵骨葆组显著高于对照组后安乐死取材。取脏器、股骨、胫骨和椎骨进行病理学分析、骨密度检测、生物力学检测、micro CT分析以及血清生化指标检测。结果实验期间各组大鼠体质量呈上升趋势,两组间比较并无统计学意义,脏器无明显病理学改变;给药6周后仙灵骨葆组全身骨密度、股骨骨密度和椎骨骨密度都显著高于对照组(P<0.05);仙灵骨葆组股骨最大载荷和屈服强度显著高于对照组(P<0.05),椎骨最大载荷也显著高于对照组(P<0.05);仙灵骨葆组胫骨骨体积分数、骨小梁厚度和骨小梁数显著高于对照组(P<0.05),骨小梁分离度显著低于对照组(P<0.05);与对照组相比,仙灵骨葆组血清OCN含量上升、TRACP-5b含量下降,差异具有统计学意义(P<0.05)。结论仙灵骨葆胶囊可能通过抑制骨吸收与促进骨形成来提高生长期大鼠骨强度,具有通过提高生长期大鼠峰值骨量来预防后期骨质疏松的潜力。Objective To study the effect of Xianling Gubao Capsule on bone strength of growing rats.Method Twenty one month old SD female rats of SPF grade were randomly divided into Control group and Xianling Gubao group,with 10 rats in each group.During the experiment,the whole body bone mineral density was measured every two weeks.After the sixth week,the Xianling Gubao group was significantly higher than the Control group,the samples were killed.The organs,femur,tibia and vertebrae were taken for pathological analysis,bone mineral density test,biomechanical test,micro CT analysis and serum biochemical indexes.Result During the experiment,the body mass of rats in each group showed an upward trend,there was no statistical significance between the two groups,and there were no obvious pathological changes in organs;After 6 weeks of administration,the BMD of whole body,femur and vertebra in Xianling Gubao group were significantly higher than those in the Control group(P<0.05);The maximum load and yield strength of femur in Xianling Gubao group were significantly higher than those in the Control group(P<0.05),and the maximum load of vertebra was also significantly higher than those in the Control group(P<0.05).The volume fraction,trabecular thickness and trabecular number of tibia in Xianling Gubao group were significantly higher than those in the Control group(P<0.05),and the separation degree of trabecular bone was significantly lower than that in the Control group(P<0.05);Compared with the Control group,the content of serum OCN increased and the content of TRACP-5b decreased in Xianling Gubao group(P<0.05).Conclusion Xianling Gubao Capsule may improve the bone strength of growing rats by inhibiting bone absorption and promoting bone formation.It has the potential to prevent late osteoporosis by increasing the peak bone mass of growing rats.

关 键 词：仙灵骨葆胶囊 生长期大鼠 骨强度 峰值骨量 

分 类 号：Q95-3[生物学—动物学]