党参多糖对脑缺血再灌注损伤大鼠血清S100-β及NSE水平的影响  

Effects of Codonopsis Pilosula Polysaccharide on Serum S100-β and NSE Levels in Rats with Cerebral Ischemia-Reperfusion Injury

作　　者：刘飞 邹赢锌 焦勇 唐瑛 LIU Fei;ZOU Yingxin;JIAO Yong;TANG Ying(Naval Characteristic Medical Center,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学海军特色医学中心,上海200433

出　　处：《实验动物科学》2023年第5期53-59,共7页Laboratory Animal Science

基　　金：高气压致轻度认知功能障碍的机制(20AH0301)。

摘　　要：目的观察党参多糖对缺血再灌注损伤大鼠血清S100-β蛋白(S100-β)及神经特异性烯醇化酶(NSE)含量的影响。方法随机将SD大鼠分成5组:假手术组、模型组、依达拉奉组和2个党参多糖高、低剂量组,每组10只。采用线栓法阻断大鼠中脑闭塞(MCAO)2 h后再灌注24 h建立脑缺血再灌注模型,2个党参多糖和依达拉奉组大鼠术前连续14 d给予相对应的药物,于再灌注后24 h取样,采用尼氏染色和HE染色法进行组织学观察,测量脑含水量及伊文思蓝法以评估卒中,ELISA法分别检测各组血清中NSE和S100-β水平。结果与假手术组比较,模型组大鼠脑组织含水量及通透性明显增加(P<0.01),血清中NSE及S100-β蛋白含量明显增加(P<0.01)。与模型组相比较,党参多糖高、低2个剂量组血清中NSE和S100-β蛋白含量明显降低(P<0.01),且党参多糖高剂量组脑组织含水量及通透性明显降低(P<0.01)。结论党参多糖能明显减轻大鼠脑缺血再灌注损伤的程度,降低血清中NSE和S100-β水平,具有良好的神经保护作用。Objective To observe Codonopsis pilosula polysaccharide(CPPS)on ischemia-reperfusion injury of rat serum S100-βprotein and neuron-specific enolase(NSE)levels.Method SD rats were randomly divided into 5 groups,namely sham operation group,model group,Edaravone group and 2 codonopsis pilosula polysaccharide(CPPS)high-dose and low-dose groups,with 10 rats in each group.By using line switch block rat middle cerebral artery occlusion(MCAO)2 h reperfusion 24 h after cerebral ischemia reperfusion model is set up,two CPPS and edaravone group rats were given corresponding drugs for 14 consecutive days before surgery,Nissl staining and HE were used for histological observation 24 h after reperfusion.Cerebral water content was measured and Evans blue method was used to assess cerebral apoplexy.Serum NSE and S100-βlevels were determined by ELISA.Result Compared with sham operation group,the water content and permeability of brain tissue in model group were significantly increased(P<0.01),and the contents of NSE and S100-βprotein in serum were significantly increased(P<0.01).Compared with model group,serum NSE and S100-βprotein contents in CPPS high-dose and low-dose groups were significantly decreased(P<0.01),and brain tissue water content and permeability in CPPS high-dose group were significantly decreased(P<0.01).Conclusion CPPS can significantly reduce the level of NSE and S100-βin serum of rats with cerebral ischemia-reperfusion injury,and have good neuroprotective effect.

关 键 词：党参多糖 脑缺血再灌注 S100-Β蛋白 神经元特异性烯醇化酶 大鼠 

分 类 号：Q95-3[生物学—动物学]