基于60K芯片的玉米回交群体精准背景选择策略  

Accurate Background Selection Strategy for Maize Backcross Population Based on 60 K-array

作　　者：朱少喜 张云龙 赵怡锟 许理文[1] 刘文彬[1] 徐翠 葛建镕[1] 霍永学 易红梅[1] 路运才[2] 王凤格[1] ZHU Shao-xi;ZHANG Yun-long;ZHAO Yi-kun;XU Li-wen;LIU Wen-bin;XU Cui;GE Jian-rong;HUO Yong-xue;YI Hong-mei;LU Yun-cai;WANG Feng-ge(Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding,Beijing 100097;School of Modern Agriculture and Ecology,Heilongjiang University,Harbin 150080,China)

机构地区：[1]北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室,北京100097 [2]黑龙江大学现代农业与生态环境学院,哈尔滨150080

出　　处：《玉米科学》2023年第4期43-51,共9页Journal of Maize Sciences

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20200305)。

摘　　要：利用Maize 6H-60K芯片对京724/京92/京92的BC_(1)代群体进行分子标记辅助背景选择,结合SNP数据特征和回交群体分离特征,开发出一套精准背景选择策略(ABSS:Accurate Background Selection Strategy)及相关算法。从统计结果中选取背景回复程度最高的一批单株作为下一代回交群体的亲本材料。基于Maize 6H-60K芯片分析和筛选产生的28910个多态性位点,可以对727个样品的背景回复程度进行精细排序,样品背景回复率大部分分布在0.30~0.75(约占样品总数的94.1%),背景回复率在0.75及以上的样品比例为1.8%。通过分析回交群体重组交换规律以及背景回复率与株高的相关性,进一步验证Maize 6H-60K芯片数据和ABSS的准确性。利用Maize 6H-60K芯片结合ABSS可以精细选择背景回复程度高、重组交换频率相对较低的单株,显著提高背景选择效率,为设计玉米回交群体规模提供参考价值。Using the Maize 6H-60K array to perform molecular marker-assisted background selection on the BC_(1)generation population of J724/J92/J92,and a set of ABSS(Accurate Background Selection Strategy)and related algorithms were developed based on SNP data characteristics and backcross population separation characteristics.Samples with high background recovery rate were selected as the parent material of the next generation backcross population.Based on the 28910 polymorphic loci generated by Maize 6H-60K array analysis and screening,the background recovery rate of 727 samples were finely sorted.The background recovery rate of samples is mostly be-tween 0.30-0.75(approximately 94.1%of the total number of samples),and the proportion of samples with back-ground recovery rate of 0.75 and above was 1.8%.The accuracy of Maize 6H-60K array data and ABSS was further verified by analyzing the recombination and exchange rules of backcross population and the correlation between background recovery rate and plant height.Using the Maize 6H-60K array combined with ABSS can select individu-al plants with a high background recovery rate and a relatively low frequency of recombination exchange,which sig-nificantly improves the efficiency of background selection,and provide reference value for designing the size of the maize backcross population.

关 键 词：玉米 Maize 6H-60K芯片 回交群体 背景选择 

分 类 号：S513.035.3[农业科学—作物学]