基于腺苷A1受体探讨益肾达络饮对EAE小鼠神经保护作用的机制  

Exploration on the neuroprotective mechanism of Yishen Daluo Decoction on EAE mice by Adenosine A1 receptor

作　　者：王响 丁文婧 吴元 王诗洋 谢名宗 何静文 尚晓玲[1] WANG Xiang;DING Wen-jing;WU Yuan;WANG Shi-yang;XIE Ming-zong;HE Jing-wen;SHANG Xiao-ling(Changchun University of Chinese Medicine,Changchun,Jilin 130117 China)

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《时珍国医国药》2023年第7期1599-1603,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81873216)。

摘　　要：目的从腺苷A1受体(A1AR)探讨益肾达络饮治疗实验性自身免疫性脑脊髓炎(EAE)的机制。方法将60只C57BL/6雌性小鼠随机分成6组,即空白组、模型组、中药组、西药组、A1AR拮抗剂1,3-二丙-8-环戊黄嘌呤(DPCPX)组、DPCPX+中药组。除空白组外,其余组均制作EAE模型。模型组、DPCPX组给予0.9%氯化钠溶液灌胃,西药组给予醋酸泼尼松混悬液灌胃,中药组、DPCPX+中药组给予益肾达络饮灌胃。通过体重、神经功能评分、病理观察、炎症因子水平,蛋白表达等观察中药对EAE的影响,分析中医治疗EAE的机制。结果益肾达络饮可以缓解EAE小鼠体重的下降,降低神经功能评分,减轻中枢神经系统炎性细胞浸润,降低血清中白介素(IL)-6、IL-17的浓度,升高中枢神经系统中A1AR及神经胶质细胞源性神经营养因子(GDNF)的表达,而DPCPX阻滞了中药的治疗作用。结论益肾达络饮的神经保护作用机制可能是通过激动中枢神经系统中A1AR活性,抑制神经炎性反应,从而发挥神经保护作用,缓解EAE的疾病状态。Objective To explore the mechanism on treatment of experimental autoimmune encephalomyelitis(EAE)from Adenosine A1 receptor(A1AR).Methods Sixty C57BL/6 female mice were randomly divided into six groups,normal control group,model group,Chinese medicine group,hormone group,8-cyclopentyl-1,3-dipropylxanthine(DPCPX)group,DPCPX+Chinese medicine group.EAE modal was made for every mice except normal control group.Model group and DPCPX group were given 0.9%sodium chloride solution,hormone group was given prednisone acetate,Chinese medicine group and DPCPX+Chinese medicine group were given Yishen Daluo Decoction.The effect of Chinese medicine on EAE was observed by weight,clinical scores,pathological observation,inflammatory factor levels,protein expression.Results The weight loss of EAE mice was alleviated,the clinical scores were reduced,the inflammatory cell infiltration of the central nervous system was reduced,the concentration of IL-6 and IL-17 in the serum was reduced and the expression of A1AR and GDNF in the central nervous system was increased by treatment of Chinese medicine.While DPCPX blocked the therapeutic effect of Chinese medicine.Conclusion The neuroprotective mechanism of Chinese medicine may be stimulating the activity of A1AR in the central nervous system,inhibit the neurological inflammatory response,exert a neuroprotective effect,and alleviate the disease state of EAE.

关 键 词：多发性硬化 实验性自身免疫性脑脊髓炎 GDNF 腺苷A1受体 IL-6 IL-17 益肾达络饮 DPCPX 

分 类 号：R285.5[医药卫生—中药学]