鸭源产尿酸氧化酶菌株的筛选及其降尿酸能力评估  

Screening of high-yield urate oxidase strain from duck and evaluation in reducing uric acid

作　　者：叶胜强 麻燕明 杨宇 童新红 李中心 钱运国 龚萍 YE Sheng-qiang;MA Yan-ming;YANG Yu;TONG Xin-hong;LI Zhong-xin;QIAN Yun-guo;GONG Ping

机构地区：[1]武汉市农业科学院,湖北武汉430208 [2]湖北洪山实验室,湖北武汉430070 [3]华中农业大学动物遗传育种与繁殖教育部重点实验室,湖北武汉430070

出　　处：《饲料研究》2023年第19期64-68,共5页Feed Research

基　　金：国家现代农业产业技术体系专项(项目编号:CARS-42-48);湖北省技术创新专项(项目编号:2022BEC037);湖北洪山实验室重大项目(项目编号:2021HSZD002)。

摘　　要：试验旨在从鸭粪便中筛选具有高产尿酸氧化酶的菌株,为缓解和治疗家禽高尿酸血症提供新的解决方案。试验从健康肉鸭新鲜粪便中筛选能在尿酸筛选培养基上产生透明圈的菌株,采用平板点种法测定菌株的产酶活性,采用16S rDNA测序法进行分子鉴定。试验菌株拌料饲喂樱桃谷鸭评定饲用效果,并评估候选菌株对高尿酸樱桃谷鸭的影响。结果显示,初步筛选出56株产尿酸氧化酶的菌株,其中1株有产蛋白酶能力、7株有产淀粉酶能力;91%的菌株为革兰氏阴性菌,对27株进行分子鉴定,15株为产碱杆菌属。菌株AS1、P56分别与粪产碱杆菌、瓜里科假单胞菌同源性最高。菌株AS1、P56对鸭平均日采食量、体重无明显影响,AS1可显著降低高尿酸血症鸭的血清尿酸水平(P<0.05)。研究表明,利用尿酸筛选培养基可从鸭粪便中筛选出高产尿酸氧化酶的菌株,AS1可作为候选菌株为后续缓解和治疗家禽高尿酸血症提供参考。The study was to screen strains with high production of urate oxidase from duck feces and provide a new solution for alleviating and treating hyperuricemia in poultry.The strain that could produce transparent circle on uric acid screening medium was screened from fresh feces of healthy meat ducks.The enzyme production activity of the strain was determined by plate spot planting method,and the molecular identification was performed by 16S rDNA sequencing.The test strain mix was fed to Cherry Valley ducks to evaluate the feeding effect,and the effect of candidate strains on high uric acid Cherry Valley ducks was evaluated.The results showed that 56 strains producing uric acid oxidase were screened,among which one strain had the ability to produce protease and seven strains had the ability to produce amylase.91%of the strains were gram negative,27 of which were identified by molecular analysis,and 15 were of the Alcaligenes faecalis.Strains AS1 and P56 had the highest homology with Alcaligenes faecalis and Pseudomonas guariconensis,respectively.Strains AS1 and P56 had no significant effect on daily feed intake and body weight of ducks.AS1 could significantly reduce the serum uric acid concentration of hyperuricemic ducks(P<0.05).The experiment indicates that using the uric acid screening medium,the high-yield urate oxidase strain can be selected from the feces of healthy meat ducks,and AS1 can be used as a candidate strain to provide a reference for the subsequent mitigation and treatment of poultry hyperuricemia.

关 键 词：鸭 尿酸氧化酶 产碱杆菌 高尿酸血症 

分 类 号：S816.7[农业科学—饲料科学]