红芪多糖对糖尿病早期大鼠视网膜Müller细胞的影响  

Effect of Hedysarum Polybotrys polysaccharides on Müller cells of retina in early diabetic rats

作　　者：张花治 金智生 刘晖 高英 罗向霞[3] 师霞 郑雪晶 刘素珍 徐国惠 ZHANG Hua-zhi;JIN Zhi-sheng;LIU Hui;GAO Ying;LUO Xiang-xia;SHI Xia;ZHENG Xue-jing;LIU Su-zhen;XU Guo-hui(ClinicalCollege of Traditional Chinese Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu Province,China;Department of Ophthalmology,Affiliated Hospital of Gansu Universityof Traditional Chinese Medicine,Lanzhou 730000,Gansu Province,China;Department of Ophthalmology,Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,Gansu Province,China)

机构地区：[1]甘肃中医药大学中医临床学院,甘肃兰州730000 [2]甘肃中医药大学附属医院眼科,甘肃兰州730000 [3]甘肃省中医院眼科,甘肃兰州730050

出　　处：《中国临床药理学杂志》2023年第19期2803-2807,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(82060854);甘肃省高等学校创新能力提升基金资助项目(2020A-082)。

摘　　要：目的研究红芪多糖(HPS)对糖尿病早期大鼠视网膜Müller细胞的调控作用及防护效应机制。方法用高糖饮食联合链脲佐菌素(STZ)腹腔注射法建立糖尿病大鼠模型。将大鼠随机分为正常组、模型组、阳性对照组(90 mg·kg^(-1)羟苯磺酸钙灌胃治疗)和低、中、高剂量实验组(50、100、200 mg·kg^(-1)HPS灌胃治疗)。于造模前、干预前、干预后每2周检测各组大鼠空腹血糖(FBG)及体质量水平,以蛋白质印迹法检测视网膜胶质原纤维酸性蛋白(GFAP)、血管内皮生长因子(VEGF)蛋白表达水平,用实时荧光定量聚合酶链反应分析大鼠视网膜GFAP、VEGF mRNA表达水平。结果干预过程中,正常组与模型组FBG水平总体稳定,其余组呈下降趋势,模型组FBG水平显著高于正常组;正常组体质量水平持续上升,干预4周后模型组体质量水平持续下降,阳性对照组与实验组体质量水平明显上升。正常组、模型组、阳性对照组和低、中、高剂量实验组的GFAP蛋白相对表达水平分别为0.08±0.02、1.92±0.06、0.20±0.01、0.88±0.09、0.73±0.02和0.58±0.01,VEGF蛋白相对表达水平分别为0.07±0.01、1.34±0.03、0.30±0.01、1.05±0.01、0.92±0.01和0.95±0.01,GFAP mRNA表达水平分别为1.01±0.17、3.09±0.18、1.63±0.14、2.80±0.07、1.88±0.14和1.75±0.10,VEGF mRNA表达水平分别为1.00±0.06、2.37±0.17、1.42±0.03、2.25±0.08、1.94±0.14和1.77±0.11。模型组的上述指标与正常组比较,差异均有统计学意义(均P<0.05);阳性对照组和中、高剂量实验组的上述指标与模型组比较,差异均有统计学意义(均P<0.05)。结论红芪多糖可以通过调控血糖、体质量水平,抑制GFAP、VEGF的表达,阻止新生血管生成,改善糖尿病早期大鼠视网膜Müller细胞的病理损害。Objective To investigate the regulatory effect and protective mechanism of Hedysarum Polybotrys polysaccharides(HPS)on Müller cells in early diabetic rats.Methods The diabetic rat model was established by high-sugar diet combined with streptozotocin(STZ)intraperitoneal injection.The rats were randomly divided into normal group,model group,positive control group(90 mg·kg^(-1),calcium dobesilate by gavage),and experimental-L,-M,-H groups(50,100,and 200 mg·kg^(-1),HPS by gavage).Fasting blood glucose(FBG)and body weight were measured before modeling,before intervention and every 2 weeks after intervention.The expression levels of retinal glial fibrillary acidic protein(GFAP)and vascular endothelial growth factor(VEGF)protein were detected by Western blotting.The expression levels of GFAP and VEGF mRNA in the retina of rats were analyzed by real-time fluorescent quantitative polymerase chain reaction.Results During the intervention,the FBG levels of the normal group and the model group were generally stable,and the other groups showed a downward trend,and the FBG level of the model group was significantly higher than that of the normal group;the body weight of the normal group continued to increase,after 4 weeks of intervention,the body weight level of the model group continued to decrease,while the body weight level of the positive control group and the experimental groups increased significantly.The expression levels of GFAP protein in normal group,model group,positive control group and experimental-L,-M,-H groups were0.08±0.02,1.92±0.06,0.20±0.01,0.88±0.09,0.73±0.02 and 0.58±0.01,respectively;VEGF protein expression levels were 0.07±0.01,1.34±0.03,0.30±0.01,1.05±0.01,0.92±0.01 and 0.95±0.01,respectively;GFAP mRNA expression levels were 1.01±0.17,3.09±0.18,1.63±0.14,2.80±0.07,1.88±0.14 and 1.75±0.10;VEGF mRNA expression levels were 1.00±0.06,2.37±0.17,1.42±0.03,2.25±0.08,1.94±0.14 and 1.77±0.11,respectively.There were significant differences in the above indicators between the model group

关 键 词：红芪多糖 糖尿病视网膜病变 MÜLLER细胞 胶质原纤维酸性蛋白 血管内皮生长因子 

分 类 号：R28[医药卫生—中药学]