蓖麻RcbZIP11基因克隆及表达特性  

Cloning and Expression Characteristics of RcbZIP11 Gene in Castor

作　　者：李艳肖 朱贵爽 张宏宇 向殿军 刘鹏[1,2] LI Yanxiao;ZHU Guishuang;ZHANG Hongyu;XIANG Dianjun;LIU Peng(College of Agriculture Life Science,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China;Key Laboratory of Ecological Agriculture of Horqin Sandy Land,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China;Tongliao Agricultural Technology Promotion Center,Tongliao,Inner Mongolia 028000,China)

机构地区：[1]内蒙古民族大学农学院,内蒙古通辽028000 [2]内蒙古民族大学科尔沁沙地生态农业国家民委重点实验室,内蒙古通辽028000 [3]通辽市农业技术推广中心,内蒙古通辽028000

出　　处：《西北植物学报》2023年第9期1478-1487,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(32060492);内蒙古自治区自然科学基金项目(2020LH03006,2022MS03057)。

摘　　要：为探究蓖麻bZIP(basic leucine zipper)基因在响应非生物胁迫应答过程中的作用,研究基于蓖麻转录组数据,克隆到1条在低温胁迫下显著上调表达的bZIP基因,并将其命名为RcbZIP11(GenBank No.OQ506490)。RcbZIP11基因的编码区序列全长492 bp,其推导163个氨基酸,预测是1个亲水性蛋白质,具有保守的bZIP_plant_GBF1结构域。蛋白质的多序列对比显示,RcbZIP11蛋白质在进化过程中相对保守,进化树分析显示RcbZIP11与麻风树JcbZIP11蛋白质亲缘关系最近。亚细胞定位显示RcbZIP11蛋白质定位于细胞核,并成功克隆出RcbZIP11启动子序列,预测结果显示,该区域拥有光响应元件、逆境响应类元件和激素诱导类元件。RcbZIP11基因在蓖麻不同组织(真叶、子叶、茎和根)中均有表达,在子叶中相对表达量最高;且该基因在逆境胁迫下(干旱、盐、低温和ABA)的根和真叶中其相对表达量均高于0 h,说明RcbZIP11基因积极地参与蓖麻的非生物胁迫响应。综上,研究为进一步探究RcbZIP11基因在蓖麻逆境条件生长过程中的功能奠定基础。In order to explore the role of bZIP(basic leucine zipper)gene in response to abiotic stress,a bZIP gene significantly up-regulated under low temperature stress was cloned based on the transcriptome data of Ricinus communis,and named as RcbZIP11(GenBank No.OQ506490).The coding region sequence of the RcbZIP11 gene was 492 bp in length,which deduced 163 amino acids.It was predicted to be a hydrophilic protein with a conserved bZIP_plant_GBF1 domain.Multiple sequence alignment showed that RcbZIP11 protein was relatively conservative during evolution.Phylogenetic tree analysis showed that RcbZIP11 had the closest relationship with JcbZIP11 protein.Subcellular localization showed that the RcbZIP11 protein was localized in the nucleus.The promoter sequence of the RcbZIP11 was successfully cloned.The prediction results showed that the region had light response elements,stress response elements,and hormone induction elements.RcbZIP11 gene was expressed in different tissues(true leaves,cotyledons,stems,and roots)of castor,and the relative expression level was the highest in cotyledons.The relative expression levels of RcbZIP11 in roots and leaves under stress(drought,salt,low temperature,and ABA)were higher than 0 h,indicating that the RcbZIP11 gene was actively involved in the abiotic stress response of castor.In summary,this study lays a foundation for further exploring the function of the RcbZIP11 gene in the growth process of castor under stress conditions.

关 键 词：BZIP 蓖麻 基因克隆 非生物胁迫 表达 

分 类 号：Q78[生物学—分子生物学] S565.6[农业科学—作物学]