肉苁蓉治疗肾阳虚有效部位筛选  

Screening of Effective Parts of Cistanches Herba for Treatment of Kidney-yang Deficiency

作　　者：苏国明 张超 华悦 程世赞 廉婧 聂紫璇 史辑[1,2] 贾天柱 SU Guoming;ZHANG Chao;HUA Yue;CHENG Shizan;LIAN Jing;NIE Zixuan;SHI Ji;JIA Tianzhu(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Innovation Center of Chinese Materia Medica Processing Technology of Liaoning Province,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药炮制技术产业创新中心,辽宁大连116600

出　　处：《中国中医药信息杂志》2023年第11期138-143,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81874345);国家中医药管理局-重点实验室研究领域的中医临床疗效提升项目(2022年);辽宁省自然科学基金面上项目(2022-MS-223)。

摘　　要：目的研究肉苁蓉不同提取部位对肾阳虚大鼠的改善作用,明确其补肾壮阳有效部位。方法采用水提醇沉法制备肉苁蓉总多糖部位,大孔吸附树脂柱富集肉苁蓉总寡糖和总苷部位。灌胃丙基硫氧嘧啶制备肾阳虚大鼠模型,分别予肉苁蓉总多糖143 mg/kg、总寡糖110 mg/kg、总苷82 mg/kg,ELISA测定各组大鼠血清三碘甲状腺原氨酸(T_(3))、甲状腺素(T_(4))、促甲状腺激素(TSH)、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、皮质醇(Cor)、促肾上腺皮质激素(ACTH)、睾酮(T)、雌二醇(E_(2))水平,计算cAMP/cGMP比值,RT-PCR测定大鼠下丘脑促甲状腺素释放激素(TRH)、促肾上腺皮质激素释放激素(CRH)mRNA表达,HE染色观察大鼠右肾组织病理改变情况。结果肉苁蓉总苷、总多糖和总寡糖对肾阳虚大鼠血清T_(3)、E_(2)、cGMP含量均表现出很好的回调作用,肉苁蓉总苷、总寡糖对肾阳虚大鼠血清T_(4)、TSH、ACTH含量及TRH、CRHmRNA表达有显著回调作用。结论肉苁蓉总苷、总多糖、总寡糖对大鼠肾阳虚症状有改善效果,其中总苷效果最佳。Objective To investigate the effects of different Cistanches Herba extracts on rats with kidney-yang deficiency;To identify the active components that may enhance the renal function.Methods Total polysaccharides from Cistanches Herba was prepared by water extraction and ethanol precipitation.Total oligosaccharides and glycosides in Cistanches Herba were enriched by macroporous resin column.The rat model of kidney-yang deficiency was induced by intragastric administration of propylthiouracil,and Cistanches Herba polysaccharide 143 mg/kg,oligosaccharide 110 mg/kg,and total glycosides 82 mg/kg were given respectively.ELISA was used to detect triodothyronine(T_(3)),thyroxine(T_(4)),thyroid stimulating hormone(TSH),cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP),hydrocortisone(Cor),adrenal cortex hormone(ACTH),testosterone(T),and E_(2)in the serum.The cAMP/cGMP ratio was calculated.RT-PCR was used to detect the levels of thyrotropin releasing hormone(TRH)and corticotropin releasing hormone(CRH)in hypothalamus.HE staining was used to observe the pathological changes in the right kidney tissue of rats in each group,and the improvement effect of each extracted effective part on kidney tissue was compared.Results Cistanches Herba total glycoside,total polysaccharide,and total oligosaccharide showed good callback effects on serum contents of T_(3),E_(2)and cGMP of rats with kidney-yang deficiency.Cistanches Herba total glycoside and total oligosaccharide showed good callback effects on T_(4),TSH,ACTH contents in serum and TRH and CRH mRNA expressions in hypothalamus.Conclusion The total glycoside,total polysaccharide,and total oligosaccharide of Cistanches Herba can improve the symptoms of kidney-yang deficiency in rats,among which the total glycoside has the most significant effect.

关 键 词：肉苁蓉 肾阳虚 总苷 总多糖 总寡糖 大鼠 

分 类 号：R285.5[医药卫生—中药学]