纳米金棒应用于肝吸虫血清循环抗原检测的研究  

Research on the Application of Gold Nanorods in the Detection of Circulating Antigens in Clonorchis sinensis Serum

作　　者：陈丽萍 廉晓丽 蔡子涵 李倩 欧阳天昭 杨毅梅 Chen Liping;Lian Xiaoli;Cai Zihan;Li Qian;Ouyang Tianzhao;Yang Yimei(Pre-clinical College,Dali University,Dali,Yunnan 671000,China;Jinci College,Shanxi Medical University,Taiyuan,030000,China;Clinical College,Dali University,Dali,Yunnan 671000,China;The First Affiliated Hospital of Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学基础医学院,云南大理671000 [2]山西医科大学晋祠学院,太原030000 [3]大理大学临床医学院,云南大理671000 [4]大理大学第一附属医院,云南大理671000

出　　处：《大理大学学报》2023年第10期27-33,共7页Journal of Dali University

基　　金：国家自然科学基金项目(81460316);云南省地方本科高校基础研究联合专项资金项目(2018FH001-092)。

摘　　要：目的:研究纳米金棒对肝吸虫病早期及低虫荷的检测效应。方法:纳米金棒标记不同浓度巯基化的肝吸虫特异性IgG抗体,用紫外分光光度计扫描纳米金棒吸收峰,观察吸收峰位移大小,优选出最佳标记浓度;用纳米金棒标记最佳浓度抗体,检测不同感染度、不同感染时间及不同稀释度的大鼠血清循环抗原。结果:纳米金棒标记20μg/mL抗体时,吸收峰位移最大为20.0 nm;肝吸虫特异性IgG抗体纳米金棒可检出50、100、200条囊蚴感染后3、10、17、31 d的血清循环抗原及稀释度为1︰400的血清循环抗原。结论:肝吸虫特异性IgG抗体纳米金棒对肝吸虫病早期及低虫荷的检测具有明显优势。Objective:To study the detection effect of gold nanorods on early and low worm burden of Clonorchis sinensis.Methods:Gold nanorods were labeled with different concentrations of thiolated Clonorchis sinensis-specific IgG antibodies.The absorption peaks of the gold nanorods were scanned using a UV spectrophotometer to observe the magnitude of the peak shift and determine the optimal labeling concentration.The gold nanorods labeled with the optimal concentration of antibodies were used to detect circulating antigens in rat serum with different infection degrees,infection times,and dilutions.Results:The maximum peak shift was 20.0 nm when the gold nanorods were labeled with 20μg/mL antibodies.The gold nanorods labeled with Clonorchis sinensis specific IgG antibodies could detect circulating antigens in rat serum at 3,10,17 and 31 d after infection with 50,100 and 200 cercariae,as well as at a dilution of 1∶400.Conclusion:Gold nanorods labeled with Clonorchis sinensis specific IgG antibodies have significant advantages in the detection of early and low worm burden of Clonorchis sinensis.

关 键 词：纳米金棒 肝吸虫 循环抗原 特异性IGG抗体 检测 

分 类 号：R383.22[医药卫生—医学寄生虫学]